Real-time 3D microscope system incorporating optical tweezers

被引：0

作者：

Hirano, Akinari ^{[1
,2
]}

Shimada, Naoya ^{[2
]}

Ikeuchi, Masashi ^{[2
]}

Ikuta, Koji ^{[1
,2
]}

机构：

[1] Department of Information Physics and Computing, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo

[2] Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo

来源：

Transactions of Japanese Society for Medical and Biological Engineering | 2014年 / 52卷

关键词：

Medical engineering; Microscopy; Single cell manipulation;

D O I：

10.11239/jsmbe.52.O-137

中图分类号：

学科分类号：

摘要：

Optical tweezers is a major technique to manipulate cells. 3D observation method of cell manipulation is required for the purpose of visualizing rotations and 3D position. However, this method is especially difficult because optical path of both optical tweezers and 3D observation will interfere. We developed a system to resolve the difficulty with using a variable-focus lens (VFL). We continuously acquire slice images having modified focal position and reconstruct them to get 3D image. The VFL changes focal position rapidly and is set at a position not to interfere the optical tweezers. We succeeded to get slice images in 30 fps and to reconstruct into 3D images in the experiment. © 2014, Japan Soc. of Med. Electronics and Biol. Engineering. All rights reserved.

引用

页码：O / 137

共 5 条

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[4]

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[5]

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