Relationship between laser-induced chlorophyll fluorescence intensity and laser power

被引：0

作者：

Yu, Haiye ^{[1
]}

Yang, Haoyu ^{[1
]}

Zhang, Lei ^{[1
]}

机构：

[1] Key Laboratory of Bionic Engineering, Ministry of Education, School of Biological and Agricultural Engineering, Jilin University

来源：

Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | 2009年 / 25卷 / SUPPL. 2期

关键词：

Chlorophyll; Fluorescence; Lasers; Mathematical models; Spectrum;

D O I：

10.3969/j.issn.1002-6819.2009.z2.047

中图分类号：

学科分类号：

摘要：

In order to meet the needs of developing laser-induced fluorescence (LIF) non-destructive detecting technique, the relationship between chlorophyll fluorescence intensity of cucumber leaf in vivo and laser power was studied by reflectance laser-induced fluorescence (LIF) spectrum analysis technique in this paper. Fluorescence spectrums, which were induced by four kinds of laser power (2.50, 5.00, 7.50, 10.00 mW) of two kinds of center wavelength 473 and 660 nm, respectively, were analyzed in the experiment. Under those conditions, fluorescence intensities of center wavelength 685 and 732 nm for different plant physiology information (chlorophyll content, water content) were calculated and analyzed by MATLAB software. The results showed that there was a very significant linear correlation between fluorescence intensity of each peak position and laser power; chlorophyll content impacted on the relationship between fluorescence intensity and laser power significantly, and a linear correlation between fluorescence intensity gradient of each peak position and chlorophyll content was found. However, that relation insignificantly correlated with water content. Based on those results a mathematical model with chlorophyll content parameter of relationship between fluorescence intensity and laser power was established. The relative square error of prediction of the model was less than 0.2%, and it exactly reflected the relationship between chlorophyll fluorescence intensity and laser power.

引用

页码：245 / 249

页数：4

共 11 条

[1]

Genty B., Briantais J.M., Baker N.R., The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence, Biochimica et Biophysica Acta, 990, pp. 87-92, (1989)

[2]

Rosema A., Snel J.F.H., Zahn H., Et al., The relation between laser-induced chlorophyll fluorescence and photosynthesis, Remote Sensing of Environment, 65, 2, pp. 143-154, (1998)

[3]

Subhash N., Wenzel O., Lichtenthaler H.K., Changes in blue-green and chlorophyll fluorescence emission and fluorescence ratios during senescence of tobacco plants, Remote Sensing of Environment, 69, 3, pp. 215-223, (1999)

[4]

Zhang Y., Zhao C., Liu L., Et al., Preliminary study on the effects of water stress on maize leaf physiological status through passive chlorophyll fluorescence detection, Transactions of the Chinese Society of Agricultural Engineering, 22, 9, pp. 39-43, (2006)

[5]

Hartmut K., Fatbardha B., Dectection of photosynthetic activity and water stress by imaging the red chlorophyll fluorescence, Plant Physiology and Biochemistry, 38, 11, pp. 889-895, (2000)

[6]

Calatayud A., Roca D., Martinez P.E., Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging, Plant Physiology and Biochemistry, 44, 10, pp. 564-573, (2006)

[7]

Ma J., Zhu Y., Yao X., Et al., Relationship between leaf nitrogen content and fluorescence parameters in Rice, Chinese Journal of Rice Science, 21, 1, pp. 65-70, (2007)

[8]

Lawrence A., James E., Elizabeth M., Et al., Fluorescence sensing systems: In vivo detection of biophysical variations in field corn due to nitrogen supply, Remote Sensing of Environment, 86, 4, pp. 470-479, (2003)

[9]

Mauromicale G., Ierna A., Marchese M., Chlorophyll fluorescence and chlorophyll content in field-grown potato as affected by nitrogen supply, genotype, and plant age, Photosynthetica, 44, 1, pp. 76-82, (2006)

[10]

Rosema A., Zahn H., Laser pulse energy requirements for remote sensing of chlorophyll fluorescence, Remote Sensing of Environment, 62, 1, pp. 101-108, (1997)

← 1 2 →