Screening and characterization of sponge-associated bacteria producing bioactive compounds anti-vibrio sp.

被引：0
作者：
Wahyudi A.T. [1 ]
Priyanto J.A. [1 ]
Maharsiwi W. [1 ]
Astuti R.I. [1 ]
机构：
[1] Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor
来源：
Wahyudi, Aris Tri (aristri2011@gmail.com) | 2018年 / Science Publications卷 / 14期
关键词：
16S rRNA; Bioactive compounds; NRPS; PKS; Sponge-associated bacteria; Vibriosis;
D O I：
10.3844/ajbbsp.2018.221.229
中图分类号：
学科分类号：
摘要：
In Indonesia, vibriosis is the main disease in shrimp. This disease is caused by Vibrio sp that may decrease the productivity of shrimp cultivation. Thus, exploration for new bioactive compounds as vibriosis biocontrol agent is necessary. Marine sponge-associated bacteria is one of many sources for bioactive compounds. The aim of this study was to screen marine sponge-associated bacteria producing anti-Vibrio sp’s bioactive compounds. Total 12 bacterial isolates (15%) of 80 isolates was isolated from marine sponges Hyrtios sp, Verongula sp. and Smenospongia sp. had anti-Vibrio sp activity in different spectra. The hemolytic assay showed that these 12 bacteria were not pathogen. Interestingly, 3 out of 4 potential isolates with the best anti-Vibrio activity have been confirmed to have genes involved in the synthesis of bioactive compounds, mainly Polyketide Synthase (PKS) and Non-Ribosomal Peptide Synthetase (NRPS) based on the occurrence of Ketosynthase (KS) and Adenilase (A) domain, respectively. Based on 16S rRNA gene, those four isolates were highly homolog to the Bacillus sp in different species and strains. Isolate coded as P2.24 was the only bacterium that had the widest spectrum of anti-Vibrio bioactive compounds against three Vibrio sp used i.e., Vibrio harveyi, V. parahaemolyticus, V. vulnificus. Consistently, an anti-Vibrio sp. activity of the P2.24 was also shown by antagonism assay using culture, supernatant and crude extract of the isolate. Our study indicates this bacterial isolate potentially to be further exploited for controlling vibriosis biologically and important for elucidation of bioactive compounds synthesized by this bacterium. © 2018 Aris Tri Wahyudi, Jepri Agung Priyanto, Wenang Maharsiwi and Rika Indri Astuti.
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页码：221 / 229
页数：8
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共 24 条
  • [1] Abubakar H., Wahyudi A.T., Yuhana M., Skrining bakteri yang berasosiasi dengan spons Jaspis sp. Sebagai penghasil senyawa antimikroba, ILMU Kelautan, 16, pp. 35-40, (2011)
  • [2] Aguirre-Guzman G., Lopez-Acevedo E.A., Vazquez-Sauceda M.D.L., Vibrio harveyi effect under survival of Litopenaeus vannamei larvae, Scientia Agropecuaria, 4, pp. 121-127, (2013)
  • [3] Boettger D., Hertweck C., Molecular diversity sculpted by fungal PKS-NRPS hybrids, Chembiochem, 14, pp. 28-42, (2013)
  • [4] Brinkmann C.M., Marker A., Kurtboke D.I., An overview on marine sponge-symbiotic bacteria as unexhausted sources for natural product discovery, Diversity, 9, pp. 40-40, (2017)
  • [5] Chatterjee S., Haldar S., Vibrio related diseases in aquaculture and development of rapid and accurate identification methods, J. Marine Sci. Res. Dev., pp. 002-002, (2012)
  • [6] Ginting E.L., Warouw V., Suleman R.W., Aktivitas antibakteri dari ekstrak kasar bakteri yang berasosiasi dengan sponge Acanthostrongylophora sp, J. Perikanan Dan Kelautan Tropis, 6, pp. 160-163, (2010)
  • [7] Hassan I., Ibrahim H.A.H., Production, characterization and valuable applications of exopolysaccharides from marine Bacillus subtilis SH1, Polish J. Microbiol., 66, pp. 449-461, (2016)
  • [8] Lee Y.K., Lee J.H., Lee H.K., Microbiol symbiosis in marine sponge, J. Microbiol., 39, pp. 254-264, (2001)
  • [9] Legina R.S., Penggunaa Ekstrak Bakteri Flavobacterium Sp. Dari Karang Acropora Muricata Sebagai Antibakteri Terhadap Vibrio Harveyi. Hasanuddin University, (2016)
  • [10] Manikandan S., Ganesapandan S., Sangeetha N., Kumaraguru A.K., Antimicrobial activity of marine sponge associated some marine bacterial metabolites against multi drug resistance human pathogens, Res. J. Microbiol., 9, pp. 25-33, (2014)
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