Fluorescent Probe Derived from 2-(2′-Hydroxyphenyl）-Benzothiazole for Hydrogen Peroxide Sensing: Synthesis, Characterization and Application

被引：0

作者：

Shi, Lei ^{[1
]}

Huang, Ling ^{[2
]}

Huang, Zejian ^{[1
]}

Li, Donghua ^{[1
]}

Gong, Shengzhao ^{[1
]}

机构：

[1] College of Light Chemical Engineering, Guangdong Polytechnic Normal University, Guangzhou

[2] Anan Cosmetics and Healthcare Products Co. ，Ltd., Foshan

来源：

Faguang Xuebao/Chinese Journal of Luminescence | 2024年 / 45卷 / 10期

关键词：

2-(2′-hydroxyphenyl）benzothiazole; bioimaging analysis; fluorescent probe; hydrogen peroxide;

D O I：

10.37188/CJL.20240181

中图分类号：

学科分类号：

摘要：

Hydrogen peroxide（H2O2），a member of small-molecule reactive oxygen species（ROS），plays vital roles in normal physiological activities and the occurrence of many diseases. In this work，a novel fluorescent probe was synthesized with the 2‐ （2′‐hydroxyaryl）benzothiazoles（HBT）as the fluorophores and 4-nitrophenyl acetate moiety as the reaction sites. The probe 1 exhibited a high sensitivity for H2O2 with the detection limit of 1. 26 μmol/L，and displayed a good selectivity for H2O2 over other reactants such as ROS，amino acids，and various ions and anions. Through high-performance liquid chromatography（HPLC）analysis，the probe’s recognition mechanism was based on the oxidation of the α-keto ester group and the hydrolysis of the ester moiety to release the fluorophore HBT. Moreover，probe 1 has low cytotoxicity and cell permeability，and was successfully applied to bioimaging analysis of H2O2 within HeLa cells，confirming its feasibility and practicality for H2O2 detection at the cellular level. © 2024 Editorial Office of Chinese Optics. All rights reserved.

引用

页码：1741 / 1746

页数：5

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