Preparation of Complex of Oats β-glucan and Quercetin and Its Effect on Lifespan of Caenorhabditis elegans

被引：0
作者：
Zhao J. [1 ]
Si X. [1 ]
Zhang K. [1 ]
Liu H. [1 ,2 ]
Guo Q. [1 ,2 ]
Wang C. [1 ,2 ]
Liang H. [3 ]
Jiang A. [4 ]
Li Z. [1 ,2 ]
机构：
[1] School of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin
[2] State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin
[3] Subtropical Crops Research Institute of Guangxi Zhuang Autonomous Region, Nanning
[4] Guilin Yuanxinda Biotechnology Co Ltd, Guilin
来源：
Journal of Food Science and Technology (China) | 2022年 / 40卷 / 03期
关键词：
Caenorhabditis elegans; Lifespan; Polysaccharide and polyphenols composites; Quercetin; Β-glucan of oats;
D O I：
10.12301/spxb202100903
中图分类号：
学科分类号：
摘要：
The covalent and non-covalent complex of β-glucan of oats and quercetin were prepared using radical catalysis and hydrogen bond adsorption methods, named COGQ and NOGQ, respectively. Their molecular structures were characterized using HPSEC (high performance size exclusion chromatography), FT-IR spectrometry and NMR (nuclear magnetic resonance) spectroscopy. The anti-aging activities of the complex were evaluated by Caenorhabditis elegans(C. elegans) model. Results from HPSEC showed that the relative molecular weights of NOGQ and COGQ were 354 kDa and 349 kDa, respectively, which were not much different. Compared with β-glucan of oats, both complexes demonstrated several new infrared absorption peaks at 1 660 cm-1, 1 243 cm-1 and 1 562 cm-1 and polysaccharide and polyphenols conjugates are amorphous. According to the NMR data, three monosaccharide residues were observed in the anomeric region of 1H spectrum and three new proton signals at 6.5-8.5 were attributed to phenyl groups in the complex. Compared with the control group, COGQ and NOGQ significantly increased the average lifespan of C. elegans (P<0.05), and the activities of SOD and CAT in both groups were significantly increased (P<0.01), suggesting that COGQ and NOGQ composites may prolong the lifespan of C. elegans by improving the antioxidant capacity of the body. The results could provide a reference for the preparation of polysaccharides and polyphenol composites and the development and utilization of them as novel food antioxidants. © 2022, Editorial Department of Journal of Food Science and Technology. All right reserved.
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页码：34 / 42and55
页数：4221
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