Enzymatic hydrolysis condition of pretreated corncob by oxalic acid to improve ethanol production

被引：2

作者：

Lim, Woo-Seok ^{[1
]}

Lee, Jae-Won ^{[1
]}

机构：

[1] Department of Forest Products and Technology, BK21 Program, Chonnam National University

来源：

Journal of the Korean Wood Science and Technology | 2012年 / 40卷 / 04期

关键词：

Enzymatic hydrolysis; Ethanol fermentation; Optimal condition; Oxalic acid pretreatment;

D O I：

10.5658/WOOD.2012.40.4.294

中图分类号：

学科分类号：

摘要：

In this study, we investigated the features of bioethanol fermentation of corncob biomass after oxalic acid pretreatment as well as enzymatic hydrolysis. The enzymatic hydrolysis was performed with Accellerase 1000 and the highest yield of monomeric sugars (64.8 g/l) was obtained at 50°C and pH 4.5 for 96 hrs hydrolysis period. For the ethanol fermentation the monomeric sugars obtained from pretreated corncob were subjected to the biological treatment using Pichia stipitis CBS 6054. It was turned out that ethanol production from oxalic acid pretreated corncob was the most feasible at 10-14% of biomass loading as well as 15 FPU enzyme amount. Under these fermentation condition, the ethanol yield was approached to 0.47 after 24 hrs fermentation period, which was corresponded to 92.2% of conversion rate.

引用

页码：294 / 301

页数：7

共 15 条

[1]

Bansal P., Hall M., Realf M.J., Lee J.H., Modeling cellulase kinetics on lignocellulosic substrate, Biotechnology Advances, 27, pp. 833-848, (2009)

[2]

Berlin A., Gilkes N., Kilburn D., Maximenko V., Bura R., Markov A., Skomarovsky A., Gusakov A., Sinitsyn A., Okunev O., Solovieva J., Saddler J.N., Evaluation of cellulase preparations for hydrolysis of hardwood substrates, Appled Biochemisty and Biotechnology, 130, pp. 528-545, (2006)

[3]

Chen M., Zhao J., Xia L., Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars, Carbohydrate Polymers, 71, pp. 411-415, (2008)

[4]

Ferreira S., Duarte A.R., Riberio M.H.L., Queiroz J.A., Domingues F.C., Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production, Biochemical Engineering Journal, 45, pp. 192-200, (2009)

[5]

Galbe M., Zacchi G., A review of the production of ethanol from softwood, Applied Microbiology Biotechnology, 59, pp. 618-628, (2002)

[6]

Gupta R., Sharma K.K., Kuhad R.C., Separate hydrolysis and fermentation (SHF) of Prosopis juliflor, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisia and Pichia stipitis-NCIM 3498, Bioresource Technology, 100, pp. 1214-1220, (2009)

[7]

Lee J.W., Rodrigues R.C.L.B., Kim H.Y., Choi I.G., Jeffries T.W., The roles of xylan and lignin in oxalic acid pretreated corncob during separate enzymatic hydrolysis and ethanol fermentation, Bioresource Technology, 101, pp. 4379-4385, (2010)

[8]

Lee J.W., Rodrigues R.C.L.B., Jeffries T.W., Simultaneous saccharification and ethanol fermentation of oxalic acid pretreated corncob assessed with response surface methodology, Bioresource Technology, 100, pp. 6307-6311, (2009)

[9]

Ramos L.P., Breuil J.N., Saddler J.N., The use of enzyme recycling and the influence of sugar accumulation on cellulose hydrolysis by Trichoderma cellulases, Enzyme and Microbial Technology, 15, pp. 91-125, (1993)

[10]

Reese E.T., Inactivation of cellulase by shaking and its prevention by surfactants, Journal of Applied Biochemistry, 2, pp. 36-39, (1980)

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