An immunosensing system for antibody detection based on capacitive micromachined ultrasound transducer resonance sensors

被引：0
作者：
机构：
[1] NanoTechnas - Center of Nanotechnology and Materials Science, Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, Vilnius
[2] Department of Electrical Engineering, Kaunas University of Technology, Panevezys Institute, Daukanto 12, Panevezys
[3] Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius
来源：
Makaraviciute, Asta | 1746年 / American Scientific Publishers卷 / 12期
关键词：
Capacitive micromachined ultrasound transducers; Gravimetric sensors; Immunosensing; Label-free biochemical detection; Sensor arrays;
D O I：
10.1166/sl.2014.3397
中图分类号：
学科分类号：
摘要：
Capacitive micromachined ultrasound transducers having a 40 MHz resonance frequency were fabricated and successfully applied to gravimetric immunosensing of specific antibodies. Bovine leukemia virus antigen gp51 was immobilized on silicon nitride coated sensor surfaces for the detection of specific antibody-horseradish peroxidase conjugates. These antibody conjugates were employed in order to increase the sensitivity of the designed immunosensing systems and to confirm the immune complex formation by a horseradish peroxidase-catalyzed enzymatic reaction. After the immune complex formation a 43 kHz resonance frequency decrease and the resistance increase of 1.35 Ω were observed. The CMUT sensor surfaces could be reverted to the initial values of the resonance frequency and resistance in approximately 6-12 min. Copyright © 2014 American Scientific Publishers
引用
收藏
页码：1746 / 1749
页数：3
相关论文
共 19 条
  • [1] Wang T.X., Hu N., Cao J.Y., Wu J.Y., Su K.Q., Wang P., Biosens. Bioelectron., 49, (2013)
  • [2] Zhou S., Zhao Y.F., Mecklenburg M., Yang D.J., Xie B., Biosens. Bioelectron., 49, (2013)
  • [3] Zhou Q., Yang L., Wang G.C., Yang Y., Biosens. Bioelectron., 49, (2013)
  • [4] Makaraviciute A., Ramanaviciene A., Biosens. Bioelectron., 50, (2013)
  • [5] Ramanavicius A., Finkelsteinas A., Cesiulis H., Ramanaviciene A., Bioelectrochemistry, 79, (2010)
  • [6] Ramanavicius A., Ryskevic N., Oztekin Y., Kausaite-Minkstimiene A., Jursenas S., Baniukevic J., Kirlyte J., Bubniene U., Ramanaviciene A., Anal. Bioanal. Chem., 398, (2010)
  • [7] German N., Kausaite-Minkstimiene A., Kirlyte J., Makaraviciute A., Ramanavicius A., Mikoliunaite L., Ramanaviciene A., Analyst, 138, (2013)
  • [8] Kausaite-Minkstimiene A., Ramanavicius A., Ruksnaite J., Ramanaviciene A., Analytical Methods, 5, (2013)
  • [9] Ramanaviciene A., German N., Kausaite-Minkstimiene A., Voronovic J., Kirlyte J., Ramanavicius A., Biosens. Bioelectron., 36, (2012)
  • [10] Balevicius Z., Ramanaviciene A., Baleviciute I., Makaraviciute A., Mikoliunaite L., Ramanavicius A., Sensors and Actuators B: Chemical, 160, (2011)
12