Cell-surface interactions of rat tooth germ cells on various biomaterials

被引：25

作者：

Chen, Rung-Shu ^{[1
]}

Chen, Yi-Jane ^{[2
,3
]}

Chen, Min-Huey ^{[2
,3
]}

Young, Tai-Horng ^{[1
]}

机构：

[1] Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University

[2] Department of Dentistry, National Taiwan University Hospital

[3] School of Dentistry, College of Medicine, National Taiwan University

来源：

Journal of Biomedical Materials Research - Part A | 2007年 / 83卷 / 01期

关键词：

Amelogenin; Collagen type I; Poly(lactic-co-glycolic acid); Polyethylene-co-vinyl alcohol; Polyvinyl alcohol; Polyvinylidene fluoride; Tooth germ cells;

D O I：

10.1002/jbm.a.31475

中图分类号：

学科分类号：

摘要：

This is the first study to explore the effect of biomaterial on tooth germ cell adhesion and proliferation in vitro. The purpose of this study is to evaluate the effects of cell-surface interactions of tooth germ cells on biomaterials with various surface hydrophilicities. The biomaterials used in this study included polyvinyl alcohol (PVA), poly(lactic-co-glycolic acid) (PLGA), poly(ethylene-co-vinyl alcohol; EVAL), and polyvinylidene fluoride (PVDF). Cell morphology was observed by photomicroscopy. Cell growth was assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity and the characteristic expression of amelogenin and collagen type I in tooth germ cells was investigated using immunocytochemistry. The results indicated that adhesion and proliferation of tooth germ cells to biomaterials with moderate hydrophilicity/hydrophobicity was superior compared to most hydrophobic material PVDF or most hydrophilic material PVA in this study. Cellular adhesion and proliferation was evident on all tested biomaterials except PVA. The cell spheroids on PVA appeared not to be proliferated and remained as well as reattachable to tissue culture plates. In conclusion, biomaterials with moderate hydrophilicity are suitable for adhesion and proliferation of tooth germ cells. The material PVA may be a good biomaterial for maintaining tooth germ cells in three-dimensional biological restoration. © 2007 Wiley Periodicals, Inc.

引用

页码：241 / 248

页数：7

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