Dynamic of Volatiles and Endogenous Hormones in Pinus massoniana Needles under Drought Stress

被引：0
|
作者：
Quan W. [1 ,3 ]
Ding G. [1 ,2 ]
机构：
[1] College of Forestry, Guizhou University, Guiyang
[2] Research Center for Forest Resources & Environment, Guizhou Province, Guiyang
[3] Key Laboratory of Mountainous Environmental Protection, Guizhou Normal University, Guiyang
来源：
Ding, Guijie | 1600年 / Chinese Society of Forestry卷 / 53期
关键词：
Drought stress; Endogenous hormones; Pinus massoniana; Volatiles;
D O I：
10.11707/j.1001-7488.20170406
中图分类号：
学科分类号：
摘要：
Objective: This study aimed to reveal the change pattern of endogenous hormones content and volatile compounds in Masson pine seedlings under drought stress, and to provide a foundation for studying the stress-resistance physiology and nursery of Masson pine. Method: The seedling volatiles, under different drought stress, were identified by headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), and the endogenous hormones were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Result: A total of 13 types of volatiles were identified with GC-MS, they belong to terpenoids, aldehydes, alcohols, and ester compounds. Nine types were identified as terpenoids, and among them, α-pinene, caryophyllene, and α-phellandrene had relatively higher content. Under drought stress, relative content of monoterpenes decreased first and then increased as the drought stress intensified, but relative content of sesquiterpenes increased first and then decreased. Drought conditions promoted the synthesis of monoterpenes. The content of ABA and ZT in needles was gradually increased with the strengthening of drought stress, but GA and IAA content was decreased gradually with the strengthening of drought stress. Conclusion: HS-SPME could be one of the effective techniques to extract the volatiles of Masson pine under drought stress condition, and GC-MS is suitable for the needle volatiles identification. Under drought stress, Masson pine needles are rich in monoterpenes and sesquiterpenes. Through altering the ratio of monoterpenes and sesquiterpenes, seedlings enhanced the drought resistance. Endogenous hormones improved the drought-resistant ability to adapt the drought stress through coordination with each other. ZT and ABA are important hormones of drought stress, and deserve further study. © 2017, Editorial Department of Scientia Silvae Sinicae. All right reserved.
引用
收藏
页码：49 / 55
页数：6
相关论文
共 39 条
  • [1] Ding G.J., Zhou Z.C., Wang Z.R., Et al., Cultivation and Utilization of Pulpwood Stand for Pinus Massoniana, pp. 1-33, (2006)
  • [2] Achotegui-Castells A., Llusia J., Hoder J.A., Et al., Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa), Acta Physiologiae Plantarum, 35, 10, pp. 3047-3058, (2013)
  • [3] Bracho-Nunez A., Knothe N.M., Costa W.R., Et al., Root anoxia effects on physiology and emissions of volatile organic compounds (VOC) under short- and long-term inundation of trees from Amazonian floodplains, Springerplus, 9, 1, pp. 1-16, (2011)
  • [4] Chen F., Tholl D., Bohlmann J., Et al., The family of terpene synthase in plants: a mid size family of genes for specialized metabolism that is highly diversified throughout the kingdom, Plant Journal, 66, 1, pp. 212-229, (2011)
  • [5] Diego N.D., Perez-Alfocea F., Cantero E., Et al., Physiological response to drought in radiata pine: phytohormone implication at leaf level, Tree Physiology, 32, 4, pp. 435-449, (2012)
  • [6] Dixon R.A., Natural products and plant disease resistance, Nature, 411, 6839, pp. 843-847, (2001)
  • [7] Fan F.H., Ding G.J., Wen X.P., Proteomic analyses provide new insights into the responses of Pinus massoniana seedlings to phosphorus deficiency, Proteomics, 16, 3, pp. 504-515, (2016)
  • [8] Gargallo-Garriga A., Sardans J., Perez-Trujillo M., Et al., Opposite metabolic responses of shoots and roots to drought, Scientific Reports, 4, (2014)
  • [9] Ha S., Vankova R., Yamaguchi-Shinozaki K., Et al., Cytokinins: metabolism and function in plant adaptation to environmental stresses, Trends in Plant Science, 17, 3, pp. 172-179, (2012)
  • [10] Hatanaka A., The biogeneration of green odour by green leaves, Phytochemistry, 34, 5, pp. 1201-1218, (1993)
1234