Three-dimensional, isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy

被引：21

作者：

Liu, Sa ^{[1
]}

Nie, Jun ^{[1
]}

Li, Yusha ^{[2
]}

Yu, Tingting ^{[2
]}

Zhu, Dan ^{[2
]}

Fei, Peng ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Opt & Elect Informat, Wuhan 430074, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Hubei, Peoples R China

来源：

JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES | 2017年 / 10卷 / 05期

基金：

美国国家科学基金会;

关键词：

Light sheet fluorescent microscopy; multi-view deconvolution; mouse brain imaging; isotropic; PLANE ILLUMINATION MICROSCOPY; WHOLE-BRAIN; RESOLUTION; RECONSTRUCTION; DEEP; ULTRAMICROSCOPY; TISSUES; EMBRYOS;

D O I：

10.1112/S1793515817130061

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a three-dimensional (3D) isotropic imaging of mouse brain using light-sheet fluorescent microscopy (LSFM) in conjunction with a multi-view imaging computation. Unlike common single view LSFM is used for mouse brain imaging, the brain tissue is 3D imaged under eight views in our study, by a home-built selective plane illumination microscopy (SPIM). An output image containing complete structural information as well as significantly improved resolution (similar to 4 times) are then computed based on these eight views of data, using a bead-guided multi-view registration and deconvolution. With superior imaging quality, the astrocyte and pyramidal neurons together with their subcellular nerve fibers can be clearly visualized and segmented. With further including other computational methods, this study can be potentially scaled up to map the connectome of whole mouse brain with a simple light-sheet microscope.

引用

页数：7

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