Effect of frequency doubled Nd:YAG laser on isoflavone of soybean seeding

被引:0
作者
Zhao, Xinle [1 ]
Jin, Lihong [1 ]
Shen, Bingjun [1 ]
Wang, Yan [1 ]
Li, Jingmei [1 ]
Tian, Jian [1 ]
机构
[1] School of Life Science and Technology, Changchun University of Science and Technology, Changchun
来源
Zhongguo Jiguang/Chinese Journal of Lasers | 2009年 / 36卷 / 10期
关键词
Biotechnology; Efficiency of photosynthesis; Isoflavone; Phenyalanine ammonia-lyase activity; Pretreatment by laser; Soybean seedling;
D O I
10.3788/CJL20093610.2728
中图分类号
学科分类号
摘要
The pullulation ratio of the soybean, the content of the isoflavone for the cotyledon and laminae, and the mean height of the soybean seedling affected by a frequency doubled Nd:YAG 532 nm laser for different power density and time are presented in this paper. The mechanism is discussed from the activity of a phenylalanine ammonia-lyase (PAL) as an initial enzyme for synthesizing the isoflavone and the efficiency of the photosynthesis. The results show that the activity of the PAL and the products of the photosynthesis such as the soluble sugar, the sucrose, and the amylum are all increased after the soybean seed being irradiated by laser, in which the best result is occurred at 15 mW/mm2 of laser for 5 mins. At the same time, the efficiency of the photosynthesis of the soybean seedling is increased, which promotes the enormous accumulation of the isoflavone.
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页码:2728 / 2733
页数:5
相关论文
共 28 条
[1]  
Feng G., Cheng H., Xu H., Et al., Application of laser mutation technology in biology breeding, Laser and Optoeectraonics Progress, 44, 5, pp. 56-61, (2007)
[2]  
Han Y., Cao Y., Chen B., Development trend of laser mutation breeding for soybean, Soybean Science, 27, 3, pp. 532-535, (2008)
[3]  
Chen H., Xu H., Lu C., Et al., A survey of studies on laser mutation breeding, Acta Laser Biology Sinica, 15, 4, pp. 436-440, (2006)
[4]  
Wan X., Survey of plant breeding by laser's induced variation, Acta Laser Biology Sinica, 5, 3, pp. 865-869, (1996)
[5]  
Zhang J., Chen Y., Wang X., Effects of CO<sub>2</sub> laser treatment on seeds germination and physiology of soybean, Acta Bot. Boreal.-Occident. Sinica, 24, 2, pp. 221-225, (2004)
[6]  
Guo J., Yin R., Xu J., Et al., Mechanism research of soybean seedling cold acclimation through protreatment of soybean seeds with He-Ne laser, Laser Technology, 27, 6, pp. 506-509, (2003)
[7]  
Ren Y., Li C., Chen J., Analysis of the physiology and biochemistry effect of laser induced mutation in the onion, J. Hebei Agricultural Sciences, 5, 4, pp. 10-13, (2001)
[8]  
Cai S., Zhao X., Lu F., The influence of He-Ne laser irradiation on the active oxygen metabolism of corn seedlings, Chinese J. Lasers, A21, 9, pp. 767-771, (1994)
[9]  
Cai S., Qi Z., Ma X., The effect of He-Ne laser irradiation on soluble protein synthesis of corn seedling, Chinese J. Lasers, A27, 3, pp. 284-288, (2000)
[10]  
Wang L., Wang L., Zhao R., Et al., Study in soybean breeding of high quality and high yield, Soybean Science, 25, 3, pp. 202-211, (2006)