One-step preparation of iron-nitrogen doped carbon dot fluorescent probe for the detection of pyruvic acid

被引：0

作者：

Shi, Xiaoran ^{[1
]}

Zhao, Hui ^{[1
]}

Zhang, Han ^{[1
]}

Li, Qunfang ^{[1
,3
]}

Lou, Fangming ^{[1
,2
,3
]}

机构：

[1] School of Chemistry and Environmental Engineering, Hubei University for Nationalities, Enshi,445000, China

[2] School of Pharmacy, Zunyi Medical University, Zunyi,563000, China

[3] Hubei Provincial Key Laboratory of Rheumatic Disease Occurrence and Intervention, Enshi,445000, China

来源：

Chinese Journal of Analysis Laboratory | 2024年 / 43卷 / 03期

关键词：

Chlorine compounds - Doping (additives) - Fluorescence - Iron - Mammals - Nanocrystals - Organic acids - Quenching - Semiconductor quantum dots;

D O I：

10.13595/j.cnki.issn1000-0720.2022.112303

中图分类号：

学科分类号：

摘要：

The new iron-nitrogen doped carbon dots (Fe, N@CDs) with a fluorescence quantum yield of about 20.8% were prepared by a one-step microwave method using phthalic acid as the precursor, triethylenediamine as the nitrogen source, and heme chloride as the iron source. The morphology, composition, and luminescence properties of the prepared Fe, N@CDs were investigated. Fe, N@CDs are capable of emitting green fluorescence of 500 nm at an excitation wavelength of 425 nm. A method for the quantitative determination of pyruvate was developed based on the quenching effect of Fe, N@CDs. The quenching of Fe, N@CDs showed a good linear relationship with pyruvate concentration in the range of 50-350 µmol/L, with a correlation coefficient of R2 = 0.998. The limit of detection (LOD) was 16.7 µmol/L. This method was successfully applied to the determination of pyruvate in fetal bovine serum proteins with recoveries ranging from 99.3%to 103.4%. © 2024, Youke Publishing Co.,Ltd. All rights reserved.

引用

页码：314 / 321