Stable nitrogen removal by anammox process after rapid temperature drops: Insights from metagenomics and metaproteomics

被引：0
作者：
Wang S. [1 ,3 ]
Ishii K. [2 ,4 ]
Yu H. [3 ]
Shi X. [1 ,5 ]
Smets B.F. [2 ]
Palomo A. [2 ]
Zuo J. [1 ,3 ]
机构：
[1] Shenzhen International Graduate School, Tsinghua University, Shenzhen
[2] Department of Environmental Engineering, Technical University of Denmark, Lyngby
[3] State Key Joint Laboratory of Environmental Stimulation and Pollution Control, School of Environment, Tsinghua University, Beijing
[4] Department of Life Science and Medical Bioscience, Waseda University, Tokyo
[5] Tsinghua-Berkeley Shenzhen Institute, Shenzhen
来源：
Bioresource Technology | 2021年 / 320卷
基金：
中国国家自然科学基金;
关键词：
Anammox; Metabolisms; Metagenomics; Metaproteomics; Nitrogen removal;
D O I：
10.1016/j.biortech.2020.124231
中图分类号：
学科分类号：
摘要：
This study investigated the impacts of rapid temperature drops on anammox process performance and the metabolism of its core microbial populations through proteomic analysis. Over a 50-day period, the temperature of an up-flow granular bed anammox reactor was stepwise decreased from 35 °C to 15 °C and resulted in repeated transient increases in effluent nitrite concentrations. At 15 °C, a nitrogen removal rate of 2.71 ± 0.23 gN/(L·d) was maintained over 100 days operation. Total AnAOB population abundance (20.9%±4.9%) and AnAOB protein abundances (75.7% ± 3.3%) remained stable with decreased temperature. Key proteins of Ca. Brocadia for nitrogen metabolism, as well as for carbohydrate metabolism and primary metabolite biosynthesis were less expressed at 15 °C than 35 °C, while several proteins of heterotrophic Chloroflexi spp. involved in carbohydrate and metabolites metabolisms were expressed to a higher degree at 15 °C. Overall, metabolism of AnAOB responded at a higher degree to low temperatures than that of heterotrophs. © 2020
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