Mechanism of Lactobacillus rhamnosus Ameliorates Chronic Alcohol-induced Liver Injury in Mice

被引：0
|
作者：
Wang Y. [1 ,2 ]
Jia D. [1 ,2 ]
Hua X. [1 ,2 ]
Liu J. [1 ,2 ]
Dai W. [1 ,2 ]
Wang Y. [1 ,2 ]
机构：
[1] College of Food science and Engineering, Jilin Agricultural University, Changchun
[2] National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun
来源：
Journal of Chinese Institute of Food Science and Technology | 2018年 / 18卷 / 05期
关键词：
Chronic alcohol-induced liver injury; Intestinal function; Lactobacillus rhamnosus B10 culture supernatant;
D O I：
10.16429/j.1009-7848.2018.05.003
中图分类号：
学科分类号：
摘要：
Gut-derived endotoxin is a critical factor in the development and progression of alcoholic liver disease (ALD). Probiotics have been used to treat animal models of alcohol-induced liver injury associated with gut leakiness and endotoxemia as well as human ALD. However, the mechanism (s) of their beneficial action are not well defined. C57 BJ/6 N mice were fed the Lieberde Carli diet containing 5% alcohol for 8 weeks. Lactobacillus rhamnosus B10 culture supernatant were supplemented in the last two weeks. B10 supplementation significantly reduced alcohol-induced endotoxemia, hepatic proinflammatory cytokines steatosis, and improved liver function, which is associated with restoration of alcohol-induced reduction in the protein levels of HIF-2α and intestinal trefoil factor ITF MUC1 and p-glycoprotein m RNA and protein expression in the ilium. This study provides a mechanistic insight for utilization of probiotics for the treatment of ALD, and suggests a critical role for intestinal hypoxia and decreased trefoil factor in the development of ALD. © 2018, Editorial Office of Journal of CIFST. All right reserved.
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页码：22 / 28
页数：6
相关论文
共 18 条
  • [1] Rao R.K., Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer, Methods Mol Biol, 447, 447, pp. 171-183, (2008)
  • [2] Rivera C.A., Bradford B.U., Seabra V., Et al., Role of endotoxin in the hypermetabolic state after acute ethanol exposure, Am J Physiol, 275, 1, pp. G1252-G1258, (1998)
  • [3] Parlesak A., Schafer C., Schutz T., Et al., Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease, J Hepatol, 32, 5, pp. 742-747, (2000)
  • [4] Kirpich I.A., Solovieva N.V., Leikhter S.N., Et al., Probiotics restore bowel flora and improve liver enzymes in human alcohol-induced liver injury: a pilot study, Alcohol, 42, 8, pp. 675-682, (2008)
  • [5] Zhong W., Mcclain C.J., Cave M., Et al., The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction, Am J Physiol Gastrointest Liver Physiol, 298, 5, pp. G625-G633, (2010)
  • [6] Madsen K., Cornish A., Soper P., Et al., Probiotic bacteria enhance murine and human intestinal epithelial barrier function, Gastroenterology, 121, 3, pp. 580-591, (2001)
  • [7] Fabia R., Ar'rajab A., Johansson M.L., Et al., The effect of exogenous administration of Lactobacillus reuteri R2LC and oat fiber on acetic acid-induced colitis in the rat, Scand J Gastroenterol, 28, 2, pp. 155-162, (1993)
  • [8] Adachi Y., Moore L.E., Bradford B.U., Et al., Antibiotics prevent liver injury in rats following long-term exposure to ethanol, Gastroenterology, 108, 1, pp. 218-224, (1995)
  • [9] Ewaschuk J.B., Dieleman L.A., Probiotics and prebiotics in chronic inflammatory bowel diseases, World J Gastroenterol, 12, 37, pp. 5941-5950, (2006)
  • [10] Miyauchi E., Morita H., Tanabe S., Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of zonula occludens-1 and myosin light-chain kinase in vivo, J Dairy Sci, 92, 6, pp. 2400-2408, (2009)
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