In vitro investigation of the effect of platelet aggregation ability on the adhesion of platelets to micro-structures

被引：0

作者：

Oota-Ishigaki A. ^{[1
]}

Maruyama O. ^{[1
]}

Masuzawa T. ^{[2
]}

Nishida M. ^{[1
]}

机构：

[1] National Institute of Advanced Industrial Science and Technology, 1-2-1 Namiki, Ibaraki, Tsukuba-city

[2] Ibaraki University, 4-12-1 Nakanarusawa, Ibaraki, Hitachi-city

来源：

Journal of Biorheology | 2021年 / 35卷 / 02期

关键词：

Antithrombogenic properties; Micro-structure; Platelet adhesion; Platelet aggregation; Platelet aggregation threshold index; Surface roughness;

D O I：

10.17106/jbr.35.49

中图分类号：

学科分类号：

摘要：

Thrombus formation on rough surfaces in cardiovascular devices implanted into patients with heart failure is a significant problem. It is, therefore, necessary to investigate the thrombus formation mechanism on rough surfaces. We have proposed a hypothesis that micro-secondary flows which are proportional to the size of micro-structure composing the rough surfaces contribute the platelet adhesion around micro-structure, that is, enhance possibility of thrombus formation. In this study, blood perfusion tests were conducted, using bovine blood with different platelet aggregation abilities, to evaluate platelet adhesion on a test piece with micro-cylinders on its surface. It was found that the platelet adhesion was sufficiently enhanced and the platelets exhibited high aggregation ability. This phenomenon depends on shear flow, and the platelets may selectively adhere to the perimeter of larger structures in the presence of microstructures of different sizes. Our results indicate that the platelet adhesion increases in proportion to size of microstructures when there is an average platelet aggregation per unit platelet (NL-PATI/PLT) ratio of over 0.002. These results can aid in designing the surface roughness of the blood contact material to help reduce the risk of thrombus formation. © Japanese Society of Biorheology 2021.

引用

页码：49 / 56

页数：7

共 21 条

[1]

Baldwin JT, Borovetz HS, Duncan BW, Et al., The national, heart, lung, and blood institute pediatric circulatory support program: A summary of the 5-year experience, Circulation, 123, pp. 1233-1240, (2011)

[2]

Cevik C, Izgi C, Dechyapirom W, Nugent K., Treatment of prosthetic valve thrombosis: rationale for a prospective randomized clinical trial, J Heart Valve Dis, 19, pp. 161-170, (2010)

[3]

Potapov EV, Stepanenko A, Krabatsch T, Hetzer R., Managing long-term complications of left ventricular assist device therapy, Curr Opin Cardiol, 26, pp. 237-244, (2011)

[4]

Ogawa S, Richardson JE, Sakai T, Ide M, Tanaka KA., High mortality associated with intracardiac and intrapulmonary thromboses after cardiopulmonary bypass, J Anesth, 26, pp. 9-19, (2011)

[5]

Zingg W, Neumann AW, Strong AB, Hum OS, Absolom DR., Effect of surface roughness on platelet adhesion under static and under flow conditions, Can J Surg, 25, pp. 16-19, (1982)

[6]

Chepurov AK, Mertsalova NN, Dubovich TI, Ifashkin GV, Chekanova VD., Effect of roughness of polymer surfaces on thrombus formation, Biomed Eng, 11, pp. 312-315, (1977)

[7]

Imachi K., Surface modification in the artificial heart, J Surface Finishing Soc Japan, 46, pp. 907-911, (1995)

[8]

Linneweber J, Dohmen PM, Kertzscher U, Affeld K, Nose Y, Konertz W., The effect of surface roughness on activation of the coagulation system and platelet adhesion in rotary blood pumps, Artif Organs, 31, pp. 345-351, (2007)

[9]

Fujisawa N, Poole-Warren LA, Woodard JC, Bertram CD, Schindhelm K., A novel textured surface for blood-contact, Biomaterials, 20, pp. 955-962, (1999)

[10]

Rose EA, Levin HR, Oz MC, Frazier OH, Macmanus Q, Burton NA, Et al., Artificial circulatory support with textured interior surfaces. A counterintuitive approach to minimizing thromboembolism, Circulation, 90, pp. 1187-1191, (1994)

← 1 2 3 →