Targeted Protein Detection Using an All-in-One Mass Spectrometry Cartridge

被引：49

作者：

Zhang, Chengsen ^{[1
]}

Glaros, Trevor ^{[2
]}

Manicke, Nicholas E. ^{[1
]}

机构：

[1] Indiana Univ Purdue Univ, Dept Chem & Chem Biol, Indianapolis, IN 46202 USA

[2] US Army Edgewood Chem Biol Ctr, BioDef Branch, BioSci Div, Aberdeen Proving Ground, MD 21010 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2017年 / 139卷 / 32期

关键词：

PAPER SPRAY; ELECTROSPRAY-IONIZATION; QUANTITATION; EXTRACTION; BIOFLUIDS; CHIP;

D O I：

10.1021/jacs.7b05571

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a simple 3D printed cartridge for mass spectrometry (MS) targeted detection of plasma proteins, including post-translational modifications (PTMs). The cartridge uses an integrated antibody enrichment column to preconcentrate the protein target as well as a novel built-in substrate to ionize the protein targets for MS detection. We show several examples of using this cartridge to perform rapid detection of clinically significant proteoforms from plasma samples.

引用

页码：10996 / 10999

页数：4

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