The microstructure evolution and the change of Burgers orientation relationship between α and β phase of TC17 titanium alloy with initial lamellar microstructure were researched by EBSD. The results show that dynamic recrystallization of β phase occurs during the hot deformation process. Meanwhile, the dynamic globularization of α phase includes the formation of subboundary and the fragmentation of α phase. The fragmentized α phase eventually transforms to globularized particle. In addition, the strict Burgers orientation relationship between α and β phase is destroyed, due to the activation of prismatic slip system and other slip or twin systems. © 2017, Science Press. All right reserved.