Hemostasis properties of silk fibroin materials under different types of hydrolysis

被引：0
|
作者：
Lei C. [1 ,2 ]
Yu L. [1 ]
Zhu H. [1 ,2 ]
Zheng T. [1 ]
Chen J. [1 ,2 ]
机构：
[1] College of Textile Science and Engineering(International Institute of Silk), Zhejiang Sci-Tech University, Hangzhou
[2] Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Hangzhou
来源：
Fangzhi Xuebao/Journal of Textile Research | 2022年 / 43卷 / 04期
关键词：
Enzyme hydrolysis; Hemostasis property; Salt hydrolysis; Silk; Silk fibroin; Types of hydrolysis;
D O I：
10.13475/j.fzxb.20210302305
中图分类号：
学科分类号：
摘要：
Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method, the silk fibroin materials were prepared via a freeze-drying method. The molecular weight, water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography, water solubility test, coagulation factor test, platelet adhesion test, enzyme-linked immunosorbent assay and rat liver injury model. The results showed that compared with the salt hydrolysis method, the enzyme hydrolysis method could endow a lower molecular weight and better water soluble, which is conducive to activating the coagulation factor XII to promote endogenous coagulation. Moreover, the platelet adhesion and platelet factor 4 content increase significantly, the amount of bleeding from the rat liver is reduced with shortened bleeding time, indicating better hemostatic effect than that of Yunnan white medicine. © 2022, Periodical Agency of Journal of Textile Research. All right reserved.
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页码：15 / 19
页数：4
相关论文
共 17 条
  • [1] YANG X, LIU W, LI N, Et al., Design and development of polysaccharide hemostatic materials and their hemostatic mechanism, Biomaterials Science, 5, 12, pp. 2357-2368, (2017)
  • [2] QIAN Lumin, ZHANG Bin, Preparation and characterization of soluble hemostatic medical cotton gauze, Journal of Textile Research, 40, 5, pp. 102-106, (2019)
  • [3] BANDYOPADHYAY A, CHOWDHURY S K, DEY S, Et al., Silk: a promising biomaterial opening new vistas towards affordable healthcare solutions, Journal of the Indian Institute of Science, 99, 3, pp. 445-487, (2019)
  • [4] YAN Jia, LI Gang, Research progress on medical textiles, Journal of Textile Research, 41, 9, pp. 191-200, (2020)
  • [5] ZHONG Hongrong, FANG Yan, BAO Hong, Et al., Preparation and properties of silk fibroin based bilayer dressing materials, Journal of Textile Research, 41, 2, pp. 13-19, (2020)
  • [6] CHEN G, WE R, HUANG X, Et al., Synthesis and assessment of sodium alginate-modified silk fibroin microspheres as potential hepatic arterial embolization agent, International Journal of Biological Macromolecules, 155, pp. 1450-1459, (2020)
  • [7] HUANG X, FU Q, DENG Y, Et al., Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo, Carbohydrate Polymers, 253, (2021)
  • [8] ZHOU F, XUE Z, WANG J., Antihypertensive effects of silk fibroin hydrolysate by alcalase and purification of an ACE inhibitory dipeptide, Journal of Agricultural and Food Chemistry, 58, 11, pp. 6735-6740, (2010)
  • [9] GUO Shaozhe, Insight into structure and molecular weight of silk fibroin for research and application, pp. 11-12, (2016)
  • [10] LAN G, LU B, WANG T, Et al., Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent, Colloids & Surfaces B: Biointerfaces, 136, pp. 1026-1034, (2015)
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