Hydrogen bonding in complexes of adenosine and 4-thiouridine: A low-temperature NMR study

被引：0

作者：

Basilio Janke, Eline M. ^{[1
]}

Dunǵer, Anita ^{[1
]}

Limbach, Hans-Heinrich ^{[1
]}

Weisz, Klaus ^{[1
]}

机构：

[1] Institut für Chemie, Freie Universität Berlin, Takustrasse 3, D-14195 Berlin, Germany

来源：

Magnetic Resonance in Chemistry | 2001年 / 39卷 / 12 SPEC. ISS.期

关键词：

Biomolecules - Chemical shift - Complexation - Nuclear magnetic resonance spectroscopy - Temperature - Temperature measurement;

D O I：

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摘要：

NMR experiments were performed on the association of the two sugar-protected nucleosides 2′-deoxy-4-thiouridine and 2′-deoxyadenosine in solution. Using an aprotic CDClF2-CDF3 solvent mixture, low-temperature measurements allowed the observation of individual hydrogen-bonded complexes in the slow exchange regime. 2D NOE experiments acquired at 128 K show the preferential formation of a ternary complex with two thiouridine nucleosides simultaneously bound at the Watson-Crick and Hoogsteen site of adenosine at all molar ratios. There is no indication of the 4-thiocarbonyl group involved as proton acceptor in a hydrogen bond to the adenine base to a significant extent. A more downfield chemical shift together with a smaller 1J(N,H) scalar coupling of the Watson-Crick-bound imino proton in specifically 3-15N-labeled thiouridine points to a stronger Watson-Crick than the Hoogsteen hydrogen bond. Copyright © 2001 John Wiley & Sons, Ltd.

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