Aqueous fractionation of mucilage and protein from Camelina sativa seeds and defatted meal

被引:0
|
作者
Evangelista, Roque L. [1 ,2 ]
Hojilla-Evangelista, Milagros P. [2 ,3 ]
Gesch, Russ W. [4 ]
Cermak, Steven C. [1 ,2 ]
Isbell, Terry A. [1 ,2 ]
机构
[1] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA
[2] ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA
[3] ARS, Plant Polymer Res Unit, 1815 N Univ St, Peoria, IL 61604 USA
[4] ARS, USDA, North Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA
关键词
Seed gums; Soluble carbohydrates; Protein extraction; Proximate composition; YELLOW MUSTARD MUCILAGE; GUMS;
D O I
10.1016/j.indcrop.2024.119560
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Camelina protein and mucilage are being investigated as coproducts in addition to the oil. It has been reported that the significant amount of mucilage (10-11 %) in the seed coat interferes with the recovery of the protein. In this work, two schemes of producing mucilage and protein fractions from camelina seeds were evaluated and proximate composition of each process stream was analyzed. In the sequential extraction process, mucilage was first separated from hydrated seeds, then oil from degummed seeds, and lastly the protein from the defatted degummed meal. In the other process, aqueous fraction rich in protein and carbohydrates were extracted simultaneously from defatted meal. Proteins were obtained by alkali solubilization and acid precipitation method. Sequential extraction produced a mucilage fraction with 15.2 % protein content and a protein isolate (90.2 % crude protein). Simultaneous aqueous extraction of the defatted meal produced a mucilage fraction with 48.9-50.2 % protein content and a protein concentrate (67.4 and 75.6 % crude protein). The whey obtained had similar protein and soluble carbohydrates contents of 24.4-26.4 % and 56.5-58.2 %, respectively. The difference in composition of the mucilage, protein and whey fractions produced by each process will determine the applications of these products.
引用
收藏
页数:8
相关论文
共 50 条
  • [31] Aqueous extraction and membrane isolation of protein from defatted Gevuina avellana
    Moure, A
    Rua, M
    Sineiro, J
    Dominguez, H
    JOURNAL OF FOOD SCIENCE, 2002, 67 (02) : 688 - 696
  • [32] PRODUCTION OF SOLUBLE ENZYMATIC PROTEIN HYDROLYSATE FROM INDUSTRIALLY DEFATTED NONDEHULLED SUNFLOWER MEAL
    PARRADO, J
    BAUTISTA, J
    MACHADO, A
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (03) : 447 - 450
  • [33] Optimization of low-abundance protein extraction and abundant protein removal from defatted soybean meal
    Liu, Ming-mei
    Qi, Bin
    Liu, Zheng-xu
    Zhan, Jin-shun
    Zhan, Kang
    Zhao, Guo-qi
    JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2017, 18 (10): : 878 - 885
  • [34] EXTRACTION OF PROTEIN GRANULATES FROM MEAL OF SUNFLOWER SEEDS AND SOYBEANS
    ELSPASS, R
    SACHSE, J
    MORL, L
    FETTE SEIFEN ANSTRICHMITTEL, 1980, 82 (08): : 333 - 333
  • [35] Effects of roasting and ultrasound-assisted enzymatic treatment of Nigella sativa L. seeds on thymoquinone in the oil and antioxidant activity of defatted seed meal
    Abolghasem, Fatemeh Mashhadi
    Kim, Ryun Hee
    Park, Sun Young
    Lim, Taehwan
    Lee, Haeseong
    Hwang, Keum Taek
    Kim, Jaecheol
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2023, 103 (13) : 6208 - 6218
  • [36] High throughput phenotyping of Camelina sativa seeds for crude protein, total oil, and fatty acids profile by near infrared spectroscopy
    Anderson, James V.
    Wittenberg, Alex
    Li, Hui
    Berti, Marisol T.
    INDUSTRIAL CROPS AND PRODUCTS, 2019, 137 : 501 - 507
  • [37] Extraction and functionality of membrane-concentrated protein from defatted Rosa rubiginosa seeds
    Moure, A
    Sineiro, J
    Domínguez, H
    FOOD CHEMISTRY, 2001, 74 (03) : 327 - 339
  • [38] PREPARATION OF PROTEIN ISOLATE AND HYDROLYSATE FROM DEFATTED SUNFLOWER SEEDS AND STUDYING THEIR CHEMICAL COMPOSITION
    Khalaf, M. N.
    Rahman, S. M. Abdul
    IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 2015, 46 (03): : 433 - 439
  • [39] Beneficial Effects of Eruca sativa Defatted Seed Meal on Visceral Pain and Intestinal Damage Resulting from Colitis in Rats
    Lucarini, Elena
    Micheli, Laura
    Pagnotta, Eleonora
    Matteo, Roberto
    Parisio, Carmen
    Toti, Alessandra
    Ferrara, Valentina
    Ciampi, Clara
    Martelli, Alma
    Testai, Lara
    Calderone, Vincenzo
    Savino, Michele
    Russo, Mario
    Pecchioni, Nicola
    Ghelardini, Carla
    Di Cesare Mannelli, Lorenzo
    FOODS, 2022, 11 (04)
  • [40] Development of protein enriched noodles using texturized defatted meal from sunflower, flaxseed and soybean
    Suresh Bhise
    A. Kaur
    Poonam Aggarwal
    Journal of Food Science and Technology, 2015, 52 : 5882 - 5889