This paper presents the design and development of 3D printed multi-band frequency selective surface (FSS) for RF shielding applications. The developed FSS significantly rejects the frequency at Wi-Fi, WiMax, and ISM/WiMax bands. The FSS has been fabricated using a 3D printed ABS substrate and metalized with a copper paint as per design. Its unit cell consists of three independent sub-geometries in which two are mostly like a concentric square loop that encircles the third one, i.e., modified Jerusalem structure. All of these sub-geometries are individually designed for the different rejection bands where their combination is optimized as a unit cell of FSS. The designed unit cell rejects the Wi-Fi, WiMax, and ISM/WiMax bands centered at 2.45 GHz, 3.5 GHz, and 5.8 GHz with attenuation level more than 35 dB. The developed FSS is a prototype of RF shielding structure to be utilized for the fabrication of an interference-free test chamber which isolates the Wi-Fi, WiMax, and ISM/WiMax interference. The design of FSS is very simple and can be printed in large scale for the development of shielding applications. © 2024, Electromagnetics Academy. All rights reserved.