Functional BOD-Ad-Cmyc@BSA complex nanosensor for Cu(II) and the detection of live E. coli

被引：2

作者：

Akrofi R. ^{[1
]}

Zhang P.-L. ^{[1
]}

Chen Q.-Y. ^{[1
]}

机构：

[1] School of the Environment and Safety Engineering, School of Chemistry and Chemical Engineering, Jiangsu University, Jingkou District, Xuefu Road, Zhenjiang

来源：

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | 2020年 / 239卷

基金：

中国国家自然科学基金;

关键词：

BSA; Cu[!sup]2+[!/sup; DNA assembly; Escherichia coli; Fluorescent method;

D O I：

10.1016/j.saa.2020.118483

中图分类号：

学科分类号：

摘要：

Escherichia coli (E. coli) is abundantly present in nature. It is generally harmless to humans but some strains have been deemed very dangerous. Therefore, as an indicator of hygienic testing, the detection of E. coli is essential. In this work, a fluorescent assembly was synthesized and characterized by spectroscopic methods. It was found that the amantadine (Ad) conjugated dye (BOD-Ad) intercalated into Cmyc G4 (aptamer) forming a non-emission assembly (BOD-Ad-Cmyc), which could be lighted-up by BSA due to the formation of fluorescence nanoparticle BOD-Ad-Cmyc@BSA. Further, BOD-Ad-Cmyc@BSA can selectively bind Cu2+ forming non-emission species BOD-Ad-Cmyc@BSA-Cu2+. E. coli can turn-on the emission of BOD-Ad-Cmyc@BSA-Cu2+ system due to the copper accumulation or reduction by E. coli. Therefore, a fluorescence method for the determination of E. coli was built. The detection limit of BOD-Ad-Cmyc@BSA-Cu2+ of E. coli is 6.3 CFU/mL. Thus, this BOD-Ad-Cmyc@BSA-Cu2+ fluorescent assembly can be used for the detection of live E. coli in the environment. © 2020 Elsevier B.V.

引用

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