Reporting and reproducibility: Proteomics of fish models in environmental toxicology and ecotoxicology

被引：0

作者：

Henke, Abigail N. ^{[1
,4
]}

Chilukuri, Srikhar ^{[2
]}

Langan, Laura M. ^{[3
,4
]}

Brooks, Bryan W. ^{[3
,4
]}

机构：

[1] Department of Biology, Baylor University Waco, TX, United States

[2] Pre-Health Studies, Baylor University Waco, TX, United States

[3] Department of Environmental Science, Baylor University Waco, TX, United States

[4] Center for Reservoir and Aquatic Systems Research (CRASR), Baylor University Waco, TX, United States

来源：

Science of the Total Environment | 2024年 / 912卷

基金：

美国国家卫生研究院;

关键词：

Bioinformatics - Chemical hazards - Design of experiments - Ecology - Mass spectrometry - Molecular biology - Statistics;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Environmental toxicology and ecotoxicology research efforts are employing proteomics with fish models as New Approach Methodologies, along with in silico, in vitro and other omics techniques to elucidate hazards of toxicants and toxins. We performed a critical review of toxicology studies with fish models using proteomics and reported fundamental parameters across experimental design, sample preparation, mass spectrometry, and bioinformatics of fish, which represent alternative vertebrate models in environmental toxicology, and routinely studied animals in ecotoxicology. We observed inconsistencies in reporting and methodologies among experimental designs, sample preparations, data acquisitions and bioinformatics, which can affect reproducibility of experimental results. We identified a distinct need to develop reporting guidelines for proteomics use in environmental toxicology and ecotoxicology, increased QA/QC throughout studies, and method optimization with an emphasis on reducing inconsistencies among studies. Several recommendations are offered as logical steps to advance development and application of this emerging research area to understand chemical hazards to public health and the environment. © 2023

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