Advances of metabolic flux regulation in microbial cell factories

被引：0
作者：
Gao C. [1 ]
Guo L. [1 ]
Hu G. [2 ]
Chen X. [1 ]
Liu L. [1 ]
机构：
[1] State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi
[2] School of Pharmaceutical Sciences, Jiangnan University, Wuxi
来源：
Huagong Jinzhan/Chemical Industry and Engineering Progress | 2021年 / 40卷 / 12期
关键词：
Metabolic flux; Microbial cell factories; Regulatory strategy; Synthetic biology; Systematic metabolic engineering;
D O I：
10.16085/j.issn.1000-6613.2021-0680
中图分类号：
学科分类号：
摘要：
With the development of metabolic engineering, many chemicals can be produced by microbial cell factories. Production of chemicals by microbial cell factories has the advantages of mild reaction conditions and environmental friendliness, and is an important way to realize sustainable production. To improve the chemicals titer, yield and productivity in microbial cell factories, traditional metabolic engineering methods mainly depend on gene overexpression or gene knockout methods to increase the metabolic flux towards the target metabolic pathway. However, due to the insufficient accuracy of metabolic flux regulation, it is easy to lead to the decline of cell production capacity. Aiming at solving the bottlenecks during carbon flux regulation in microbial cell factories, this review systematically summarizes the latest progress from four perspectives: target selection of metabolic flux regulation, carbon flux balance between cell growth and chemicals synthesis, competition between by-product pathway and product synthesis, and pathway efficiency enhancement in product synthesis. The future development trend of building microbial cell factory is also prospected from the design of high-accuracy, bionic, intelligent, multi-task and quick-response tools. © 2021, Chemical Industry Press Co., Ltd. All right reserved.
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页码：6807 / 6817
页数：10
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