Methane oxidizing capacity and coal adsorbing characteristics of microbial flora

被引：0
|
作者：
Zhou Y. [1 ,2 ]
Zhang R. [1 ]
Wei J. [2 ]
Tian K. [1 ]
Gong W. [1 ]
Zhao S. [3 ]
Shi H. [1 ]
机构：
[1] School of Resources and Safety Engineering, Henan University of Engineering, Henan, Zhengzhou
[2] School of Safety Science and Engineering, Henan Polytechnic University, Henan, Jiaozuo
[3] School of Energy Science and Engineering, Henan Polytechnic University, Henan, Jiaozuo
来源：
Natural Gas Industry | 2023年 / 43卷 / 12期
关键词：
Consumption characteristics; Growth characteristics; Methane degradation rate; Methane emission reduction; Methane oxidation; Methane-oxidizing bacteria; Wetting angle;
D O I：
10.3787/j.issn.1000-0976.2023.12.016
中图分类号：
学科分类号：
摘要：
Methane-oxidizing bacteria gets energy by consuming methane, so it has wide application value in environmental methane emission reduction, but its application in coal mines underground is still in the stage of initial research. In order to promote the application of microbial technologies in coal mines and improve the treatment effect of coalbed methane, this paper selects microbial flora from the natural environment and acclimates them. Then, the microenvironmental change and gas consumption characteristics of the bacterial solution during the growth process are monitored with the experimental culture device, and the dynamic change of contact angle of pure water, culture medium and bacterial solution on the surface of different coal samples is investigated by means of contact angle measuring device and theoretical analysis. Finally, the influences of minerals in QC and XA coal samples, the former anthracite coal from Jincheng coal mine in Shanxi province and the latter coking coal from Xin'an Coal Mine in Henan Province, on wetting angle are analyzed by virtue of Fourier Transform Infrared Spectroscopy (FTIR). And the following research results are obtained. First, the selected microbial flora has strong methane oxidizing capacity. The results of two groups of experiment indicate that the methane degradation rate can reach 71.19% and 84.63% within 15 days, and the oxygen reduction rate can reach 100%. Second, the OD600 value of microbial growth density in the bacterial solution increases by 30 times, and the growth is the most vigorous from the 6th day to the 8th day. The pH value of bacterial solution increases from 6.32 to 7.25, and the ORP decreases from 210 mV to 93 mV. Third, compared with pure water and culture medium, bacterial solution has stronger wettability on the surface of coal and the smallest equilibrium contact angle on the surface of two kinds of coal samples. XA coal samples contain many hydrophilic minerals, and their equilibrium contact angles in three kinds of solution are smaller than that of QC coal samples. In conclusion, methane-oxidizing bacteria flora has high adaptability and application value in coal mines underground, indicating that microbial technologies have certain advantages in coal mine methane prevention and control and are of great significance for the safe production of coal mines. © 2023 Natural Gas Industry Journal Agency. All rights reserved.
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页码：152 / 160
页数：8
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