Statistical Optimization of Culture Medium for Enhanced Lipase Production by Aureobasidium pullulans Using Response Surface Methodology

被引：0

作者：

Zhao M. ^{[1
,2
]}

Cai H. ^{[1
,2
]}

Li Y. ^{[1
,2
]}

Lai J. ^{[1
,2
]}

Zhang H. ^{[1
,2
]}

Feng F. ^{[1
,2
]}

机构：

[1] College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou

[2] Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou

来源：

Journal of Chinese Institute of Food Science and Technology | 2017年 / 17卷 / 07期

关键词：

Aureobasidium pullulans; Lipase; Plackett-Burrman design; Response surface methodology;

D O I：

10.16429/j.1009-7848.2017.07.011

中图分类号：

学科分类号：

摘要：

A lipase producing strain, Aureobasidium pullulans S4, was isolated in privious study. To enhance the extracellular lipase production by A. pullulans, response surface methodology (RSM) was used to optimize fermentation culture medium. Firstly, a Placett-Burman design was employed to evaluate the effects of different culture medium components, which were selected by optimization experiments of preliminary single variable. Among the seven variables, sucrose, sunflower oil and NaCl were found to have significant effects on lipase production. The concentrations of these three variables were optimized using a central composite design. The optimum culture medium containing 2.14% sucrose, 4.24% sunflower oil (V/V) and 0.03% NaCl led to 3.41-fold increase in lipase production (31.75 U/mL) compared with the original medium. © 2017, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：77 / 85

页数：8

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