Effect of Thiosulfate on the Carbon Fixation Capability of Thiobacillus thioparus and Its Mechanism

被引：0
|
作者：
Li H. [1 ]
Wang L. [1 ]
Wang Y.-N. [1 ]
机构：
[1] State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai
来源：
Wang, Lei (celwang@tongji.edu.cn) | 1600年 / Science Press卷 / 38期
关键词：
CO[!sub]2[!/sub] assimilation; Electron donors; Inhibition effect of organic carbon; RubisCO;
D O I：
10.13227/j.hjkx.201610043
中图分类号：
学科分类号：
摘要：
The effect of Na2S2O3·5H2O on the carbon fixation capability of Thiobacillus thioparus(DSM 505) was determined by measuring the total organic carbon (TOC) concentrations under different concentrations of Na2S2O3·5H2O. In addition, the mechanism was clarified by analyzing the transcription characteristics of RubisCO-encoding genes (cbb genes) and the concentrations of extracellular free organic carbon (EFOC) under different concentrations of Na2S2O3·5H2O. The result showed that by increasing the concentrations of Na2S2O3·5H2O appropriately, the carbon fixation capability of Thiobacillus thioparus could be promoted and the ratio of extracellular free organic carbon to total organic carbon fell significantly. Moreover, the analysis from the transcription characteristics of cbb genes revealed that Na2S2O3·5H2O had no significant effect on the transcription efficiency and transcription pattern of cbb genes. So Na2S2O3·5H2O might improve the carbon fixation capability by promoting the cytoskeleton synthesis rate as electron donors to eliminate the inhibition effect of extracellular free organic carbon on the carbon fixation of Thiobacillus thioparus. © 2017, Science Press. All right reserved.
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页码：2496 / 2501
页数：5
相关论文
共 23 条
  • [1] Ono E., Cuello J.L., Design parameters of solar concentrating systems for CO<sub>2</sub>-mitigating algal photobioreactors, Energy, 29, 9-10, pp. 1651-1657, (2004)
  • [2] Hu J.J., Wang L., Li Y.L., Et al., Breeding, optimization and community structure analysis of non-photosynthetic CO<sub>2</sub> assimilation microbial flora, Environmental Science, 30, 8, pp. 2438-2444, (2009)
  • [3] Johnson E.J., Peck H.D., Coupling of phosphorylation and carbon dioxide fixation in extracts of Thiobacillus thioparus, Journal of Bacteriology, 89, 4, pp. 1041-1050, (1965)
  • [4] Bassham J.A., Benson A.A., Calvin M., The path of carbon in photosynthesis, The Journal of Biological Chemistry, 185, 2, pp. 781-787, (1950)
  • [5] Mei Y., Li H.L., Xie J., Et al., Riulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco), Plant Physiology Communications, 43, 2, pp. 363-368, (2007)
  • [6] Delwiche C.F., Palmer J.D., Rampant horizontal transfer and duplication of rubisco genes in eubacteria and plastids, Molecular Biology and Evolution, 13, 6, pp. 873-882, (1996)
  • [7] Yuan H.Z., Qin H.L., Liu S.L., Et al., Advances in research of molecular ecology of carbon fixation microorganism, Scientia Agricultura Sinica, 44, 14, pp. 2951-2958, (2011)
  • [8] Yuan Y., Zhou W.L., Wang H., Et al., Study on sulfur-based autotrophic denitrification with different electron donors, Environmental Science, 34, 5, pp. 1835-1844, (2013)
  • [9] Frattini C.J., Leduc L.G., Ferroni G.D., Strain variability and the effects of organic compounds on the growth of the chemolithotrophic bacterium Thiobacillus ferrooxidans, Antonie van Leeuwenhoek, 77, 1, pp. 57-64, (2000)
  • [10] Liu X.M., Lin J.Q., Tian K.L., Et al., Metabolism of organic compounds by extremely acidophilic, obligately chemolithoautotrophic thiobacilli-a review, Chinese Journal of Biotechnology, 24, 1, pp. 1-7, (2008)
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