Cytochrome P450 CYP6AE70 Confers Resistance to Multiple Insecticides in a Lepidopteran Pest, Spodoptera exigua

被引：4

作者：

Hu, Bo ^{[1
,2
]}

Xing, Zhiping ^{[1
,2
]}

Dong, Hui ^{[1
,2
]}

Chen, Xiangzhu ^{[3
]}

Ren, Miaomiao ^{[4
]}

Liu, Kuitun ^{[5
]}

Rao, Cong ^{[5
]}

Tan, Anjiang ^{[1
,2
]}

Su, Jianya ^{[5
]}

机构：

[1] Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

[2] Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Peoples R China

[3] Linyi Univ, Sch Med, Linyi 276000, Peoples R China

[4] Jiangsu Univ Sci & Technol, Coll Environm & Chem Engn, Zhenjiang 212100, Peoples R China

[5] Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2024年 / 72卷 / 42期

基金：

中国国家自然科学基金;

关键词：

Spodoptera exigua; CYP450; insecticide resistance; resistance management; CYP6AE; DROSOPHILA-MELANOGASTER; HUBNER LEPIDOPTERA; IMIDACLOPRID RESISTANCE; HELICOVERPA-ARMIGERA; BROWN PLANTHOPPER; GENES; EXPRESSION; NOCTUIDAE; ADAPTATION; EVOLUTION;

D O I：

10.1021/acs.jafc.4c04872

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Cytochrome P450 monooxygenases are associated with the detoxification of xenobiotics, and overexpression of P450 genes has been proven to be associated with insecticide resistance in insect species. Our previous study has revealed that multiple CYP6AE genes were significantly overexpressed in a resistant strain of Spodoptera exigua, and among these genes, CYP6AE70 was particularly highly expressed. However, the functional roles of the CYP6AE genes in insecticide resistance remain unknown in this pest. Here, we investigate the relationship between the CYP6AE genes and insecticide resistance by focusing on CYP6AE70. The expression of CYP6AE70 was increased after exposure to chlorpyrifos, cypermethrin, and deltamethrin. Ectopic overexpression of P450 in transgenic flies by the GAL4/UAS system dramatically enhanced the tolerance to these three insecticides. Furthermore, the recombinant CYP6AE70 was functionally expressed in Sf9 cells, and metabolic assays revealed that the recombinant P450 protein could efficiently metabolize chlorpyrifos, cypermethrin, and deltamethrin. Finally, molecular modeling and docking also showed that this P450 protein were tightly bound to the three insecticides. These results determine that the upregulation of CYP6AE genes results in resistance to multiple insecticides in S. exigua. This study improves our understanding of P450-mediated insecticide resistance and will help us to design more effective resistance management for pest control.

引用

页码：23141 / 23150

页数：10

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