Analysis of Microbial Diversity in a Fluidized-Sand Biofilter Based on High-Throughput Sequencing Technology

被引：2
|
作者：
Zhang H.-G. [1 ]
Song H.-Q. [1 ]
Gu C.-C. [1 ]
Shan J.-J. [1 ]
Zhang Y.-L. [1 ]
Ni Q. [1 ]
机构：
[1] Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai
来源：
Huanjing Kexue/Environmental Science | 2017年 / 38卷 / 08期
关键词：
Fluidized-sand biofilter; High-throughput sequencing; Microbial diversity; Microglass beads; Performance of purifying effluent;
D O I：
10.13227/j.hjkx.201702089
中图分类号：
学科分类号：
摘要：
In order to understand the microbial community composition and mechanism of purifying aquaculture water with a biofilter that was been applied to spotted parrotfish recirculating aquaculture systems with microglass beads as the biofilter filler, the changes in the nutrients of different biological filter beds and the water treatment performance of a fluidized-sand biofilter were analyzed. By using high-throughput sequencing technology, the microbial community structure of the surface area and bottom area at different times were studied. The results show that the fluidized-sand biofilter successfully start-up after one month under marine culture. Nitrification of the biofilter occurred mainly in the lower bed layer; the surface area played a small role in eliminating the NH4+-N. The average removal rates of NH4+-N, TN, BOD5, and SS were 68.3%±2.24%, 49.54%±3.56%, 60.35%±4.98%, and 45.21%±2.11%, respectively. The average NH4+-N removal load reached (343.28±75.5) g·(m3·d)-1, which showed a better performance of wastewater treatment. In this experiment, 31 phyla and 490 genera were identified, thus the biological diversity was significantly higher than that of the other conventional microbial biofilter. Start-stop cycling of the self-cleaning device in the biofilter had no effect on the microbial diversity of different regional, but had some effect on the dominant bacterial community of the microglass beads surface. Under opening self-cleaning device regularly condition, the microbial species of the surface and bottom areas became significantly similar. With time, the microbial species of the superficial area and bottom area had no significant change, and the microbial population structure was relatively stable. In the biofilm training period, the diversity of microbial communities was significantly lower than it was after biofilm maturation. At the time of biofilter stable operation, the dominant bacteria of the superficial zone essentially remained unchanged and mainly included Anaerolineaceae_uncultured (8.4%-28%), Flavobacteriaceae_unclassified (1.1%-32%), Rhodobacteraceae_uncultured (2.3%-17%), Nitrospira(1%-7%), and Caldilineaceae_uncultured (1%-6%). The dominant bacteria of the bottom zone changed over time and mainly include Nitrospira (12.45%-17.06%), Candidatus_Microthrix (2.6%-8.8%), Muricauda (4.8%-6.3%), Defluviimonas (6%-7%), and Rhodobacteraceae_uncultured (2%-6.2%). Among them, the abundance of Nitrospira was significantly higher than that in the other biofilter. © 2017, Science Press. All right reserved.
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页码：3330 / 3338
页数：8
相关论文
共 30 条
  • [1] Tsukuda S., Christianson L., Kolb A., Et al., Heterotrophic denitrification of aquaculture effluent using fluidized sand biofilters, Aquacultural Engineering, 64, pp. 49-59, (2015)
  • [2] Zhang H.G., Zhang Y.L., Zhang Y.W., Et al., Performance of purifying effluent and nitrification kinetics of fluidized-sand biofilter in recirculating aquaculture system, Chinese Journal of Environmental Engineering, 8, 11, pp. 4743-4750, (2014)
  • [3] Summerfelt S.T., Design and management of conventional fluidized-sand biofilters, Aquacultural Engineering, 34, 3, pp. 275-302, (2006)
  • [4] Liu Y., Song X.F., Liang Z.L., Et al., Application of CFD modeling to hydrodynamics of cyclobio fluidized sand bed in recirculating aquaculture systems, Journal Ocean University of China, 13, 1, pp. 115-124, (2014)
  • [5] Liu Y., Song X.F., Liang Z.L., Et al., Study on fluidization characteristics of Cyclo-Bio fluidized sand biofilters in aquaculture recirculating system, Periodical of Ocean University of China, 44, 1, pp. 41-45, (2014)
  • [6] Zhang H.G., Wu F., Zhang Y.L., Et al., Hydraulic characteristics of swirling fluidized-sand biofilter, Transactions of the Chinese Society of Agricultural Engineering, 28, 18, pp. 69-74, (2012)
  • [7] Zhang C.Y., Wang L., Yan N., Et al., Air-lift internal loop biofilm reactor for realized simultaneous nitrification and denitrification, Bioprocess and Biosystems Engineering, 36, 5, pp. 597-602, (2013)
  • [8] Li H., Huang S.B., Wei Z.D., Et al., Performance of a new suspended filler biofilter for removal of nitrogen oxides under thermophilic conditions and microbial community analysis, Science of the Total Environment, 562, pp. 533-541, (2016)
  • [9] Michaud L., Lo Giudice A., Troussellier M., Et al., Phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system, Journal of Applied Microbiology, 107, 6, pp. 1935-1946, (2009)
  • [10] Tal Y., Watts J.E.M., Schreier H.J., Anaerobic ammonium-oxidizing (Anammox) bacteria and associated activity in fixed-film biofilters of a marine recirculating aquaculture system, Applied and Environmental Microbiology, 72, 4, pp. 2896-2904, (2006)
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