Visualization of interchromatin granules in the cell nucleus by atomic force microscopy

被引：0
作者：
Zamora-Cura, Alma Leticia [1 ]
Jiménez-García, Luis Felipe [2 ]
机构：
[1] Facultad de Medicina, Ciencias Biomédicas, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacán, México D.F.
[2] Laboratorio de Nanobiología Celular, Departamento de Biología Celular, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacán, México D.F.
来源：
Journal of Advanced Microscopy Research | 2014年 / 9卷 / 04期
关键词：
Atomic force microscopy; Cell nucleus; Interchromatin granule clusters; RNP; Speckles;
D O I：
10.1166/jamr.2014.1224
中图分类号：
学科分类号：
摘要：
The cell nucleus is a highly compartmentalized and dynamic organelle involved in several activities related to gene expression. Speckles are among those compartments observed by fluorescence microscopy. Speckles contain splicing factors involved in pre-mRNA processing. When observed by transmission electron microscopy, speckles are composed of fibers and 25-30 nm in diameter individual granules. Here we have used the atomic force microscopy to analyze speckles at the nanoscale. HepG2 cells were processed as for electron microscopy and semithin sections were observed with an atomic force microscope working under contact or tapping modes. Clusters of fibers and granules about around 20-30 nm in diameter were observed in the interchromatin space. Compared to chromatin and nucleolus, these areas display different profiles. We suggest that interchromatin material correspond to interchromatin granule clusters. Copyright © 2014 American Scientific Publishers All rights reserved.
引用
收藏
页码：296 / 300
页数：4
相关论文
共 19 条
  • [1] Spector D.L., Annual Rev. Cell Biol., 9, (1993)
  • [2] Spector D.L., Cell, 127, (2006)
  • [3] Handwerger K.E., Gall J.G., Cell Biol., 16, (2006)
  • [4] Monneron A., Bernhard W., J. Ultrastruct. Res., 27, (1969)
  • [5] Vazquez-Nin G.H., Echeverria O.M., Eur. J. Histochem., 40, (1996)
  • [6] Spector D.L., Lamond A.I., CSH Persp. Biol., 1, (2011)
  • [7] George-Tellez R., Segura-Valdez M.L., Gonzalez-Santos L., Jimenez-Garcia L.F., Biol. Cell, 94, (2002)
  • [8] Albiez H., Cremer M., Tiberi C., Vecchio L., Schermelleh L., Dittrich S., Kupper K., Joffe B., Thormeyer T., Von Hase J., Yang S., Rohr K., Leonhardt H., Solovei I., Cremer C., Fakan S., Cremer T., Chromosome Res., 14, (2006)
  • [9] Schneider R., Grosschedl R., Genes Dev., 21, (2007)
  • [10] Saitoh N., Sakamoto C., Hagiwara M., Agredano-Moreno L.T., Jimenez-Garcia L.F., Nakao M., Mol. Biol. Cell, 23, (2012)
12