Spectrophotometric method for estimation of Alkaloids precipitable with dragendorff's reagent in plant materials

被引：0
|
作者：
Sreevidya, Narasimhan [1 ]
Mehrotra, Shanta [1 ]
机构：
[1] Natl. Botanical Research Institute, 2, Rana Pratap Marg, Lucknow, 226 001, India
来源：
| 2003年 / AOAC International卷 / 86期
关键词：
Agents - Bismuth compounds - Composition effects - Drug products - Organic compounds - Precipitation (chemical) - Sampling - Spectrophotometry;
D O I：
10.1093/jaoac/86.6.1124
中图分类号：
学科分类号：
摘要：
A rapid, easy, and simple spectrophotometric method was developed for the estimation of total alkaloids precipitated by Dragendorff s reagent (DR) in plant materials. It is based on the formation of yellow bismuth complex in nitric acid medium with thiourea. The yellow-colored complex formed obeys Lambert-Beer's law in the concentration range of 0.06-50 μg/mL with λmax at 435 nm. Using this method, the alkaloidal percentage of certain alkaloids (ajamalicine, papaverine, cinchonine, piperine, berberine) and some plant materials containing alkaloids (Berberis aristata, Solarium nigrum, and Piper longum) were determined. The method was compared with other methods. It can be used for routine analysis of commercial samples by industries dealing with herbal drugs for standardization of plant materials containing alkaloids and for alkaloid-containing pharmaceutical products.
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