Inhibitory Effect of Tea Polyphenols on the Pathogenicity of Aeromonas hydrophila

被引：0
|
作者：
Zhang D. [1 ]
Yan T. [1 ,2 ]
Li J. [1 ]
Yang Q. [2 ]
Ai X. [2 ]
Dong J. [2 ]
机构：
[1] College of Food Science and Engineering, Bohai University, Liaoning, Jinzhou
[2] Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan
来源：
Journal of Chinese Institute of Food Science and Technology | 2024年 / 24卷 / 01期
关键词：
aerolysin; Aeromonas hjdrophila; anti—virulence; aquatic products; tea polyphenols;
D O I：
10.16429/j.1009-7848.2024.01.003
中图分类号：
学科分类号：
摘要：
The purpose of this study was to screen new drugs that can inhibit the pathogenesis of Aeromonas hydrophila and eliminate infections caused by A, hydrophila contaminated aquatic products. The results showed that tea polyphenols had no role on bacterial growth at concentrations lower than 128 |JLg/mL, but tea polyphenols could inhibit the hemolytic activity of aerolysin at concentrations ranging from 1—8 fXg/mL. The results of thermal shift and heptamer formation assays showed that tea polyphenols could inhibit the formation of functional heptamer by directly binding to aerolysin and results in loss of activity. Cell viability assays showed that tea polyphenols of 4 u, g/mL and 8 u, g/mL could provide a significant protection to A549 cells from aerolysin mediated cell injury. Moreover, experimental therapeutics found that tea polyphenols at a dosage of 50 mg/kg could increase the survival rate of channel catfish infected with A. hjdrophila to 60%. The above experiments showed that tea polyphenols directly bind to aerolysin and inhibit the activity of aerolysin by reducing the formation of functional heptamer, and tea polyphenols can significantly reduce the pathogenicity of A. hjdrophila both in vitro and in vivo. This study suggests that tea polyphenols can be used as an alternative or adjuvant therapy to antibiotics for the treatment of A. hydrophila-ielated infections. © 2024 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：21 / 30
页数：9
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