Near-infrared Fluorescent Probe Based on Methylene Blue for Specific Detection of Hypochlorous Acid

被引：0
作者：
Yao S.-F. [1 ]
Yao Y.-S. [1 ]
Zheng W.-B. [1 ]
Ye C.-Z. [1 ]
Ying J. [1 ]
Lyu G.-L. [1 ]
Li C.-X. [1 ]
机构：
[1] College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua
来源：
Faguang Xuebao/Chinese Journal of Luminescence | 2020年 / 41卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Fluorescent probes; Hypochlorous acid; Methylene blue; Near-infrared; Reactive oxygen species;
D O I：
10.37188/fgxb20204107.0791
中图分类号：
学科分类号：
摘要：
Hypochlorous acid (HOCl) is a reactive oxygen species produced by hydrogen peroxide and chloride ions under the catalysis of myeloperoxidase (MPO). HOCl plays an important role in immune defense against pathogens. Therefore, it is of great significance to sense and detect HOCl. There are several methods for the detection of HOCl, such as electroanalysis, chromatography, chemiluminescence and fluorescence analysis and so on. Currently, fluorescence imaging technique has attracted many researchers' interest because of its advantages, including high selectivity and sensitivity, easy operation, quick response, and real-time detection. In this paper, a methylene blue-based near-infrared fluorescence probe MB-1 was designed and synthesized for the fast and specific detection of HOCl. The probe can specifically detect HOCl in vitro, accompanied by a significant enhancement of near-infrared fluorescence intensity and a remarkable colour change from colorless to blue. Moreover, the probe displayed high sensitivity towards HOCl and limit of detection (LOD) was as low as 8.2 nmol/L. In addition, the probe showed good anti-interference ability, indicating that it has the potential to detect HOCl at a physiological level. © 2020, Science Press. All right reserved.
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页码：791 / 799
页数：8
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