Metabolic engineering of corynebacterium glutamicum for acetoin production

被引：0

作者：

机构：

[1] School of Chemical Engineering and Technology, Tianjin University, Tianjin

[2] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin

来源：

Chen, Tao | 1600年 / Tianjin University卷 / 47期

关键词：

Acetoin; Acetolactate decarboxylase; Acetolactate synthase; Corynebacterium glutamicum; Metabolic engineering;

D O I：

10.11784/tdxbz201304053

中图分类号：

学科分类号：

摘要：

The acetoin was synthesized using metabolic engineering method from Corynebacterium glutamicum ATCC 13032. The alsSD operon derived from Bacillus subtilis 168 was expressed in C. glutamicum ATCC 13032 to yield acetoin of 2.14 g/L in the engineering strain. Additional inactivation of aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and ldhA gene encoding NAD+-dependent L-lactate dehydrogenase increased the supply of acetoin precursor. The acetoin production increased to 5.09 g/L with the newly constructed strain. Subsequently, formation of 2, 3-butanediol was inhibited by deleting but A gene encoding for 2, 3-butanediol dehydrogenase. Total yield of acetoin reached 8.33 g/L (51.1% of theoretical yield) under the optimum fermentation condition in the strain CGL3.The experimental results show that the strain of C. glutamicum ATCC 13032 engineered metabolically can provide a potential application for acetoin synthesis. ©, Tianjin University. All right reserved.

引用

页码：967 / 972

页数：5

共 14 条

[1]

Liu Y.F., Zhang S.L., Yong Y.C., Et al., Efficient production of acetoin by the newly isolated Bacillus licheniformis strain MEL09, Process Biochemistry, 46, 1, pp. 390-394, (2011)

[2]

Leuchtenberger W., Huthmacher K., Drauz K., Biotechnological production of amino acids and derivatives: Current status and prospects, Applied Microbiology and Biotechnology, 69, 1, pp. 1-8, (2005)

[3]

Jiang C., Wang T., Zhang Y., Et al., Breeding cadmium-resistant strains by genome shuffling and bioremediation of cadmium contaminated soil, Journal of Tianjin University, 45, 7, pp. 609-614, (2012)

[4]

Blombach B., Schreiner M.E., Holatko J., Et al., Lvaline production with pyruvate dehydrogenase complexdeficient Corynebacterium glutamicum, Applied and Environmental Microbiology, 73, 7, pp. 2079-2084, (2007)

[5]

Wieschalka S., Blombach B., Eikmanns B.J., Engineering Corynebacterium glutamicum for the production of pyruvate, Applied Microbiology and Biotechnology, 94, 2, pp. 449-459, (2012)

[6]

Zhu N.Q., Xia H.H., Wang Z.W., Et al., Engineering of acetate recycling and citrate synthase to improve aerobic succinate production in Corynebacterium glutamicum, PLoS One, 8, 4, (2013)

[7]

Inui M., Kawaguchi H., Murakami S., Et al., Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions, Journal of Molecular Microbiology and Biotechnology, 8, 4, pp. 243-254, (2004)

[8]

Yang Y.T., Peredelchuk M., Bennett G.N., Et al., Effect of variation of Klebsiella pneumoniae acetolactate synthase expression on metabolic flux redistribution in Escherichia coli, Biotechnology and Bioengineering, 69, 2, pp. 150-159, (2000)

[9]

Fu J., Wang M., Liu W., Et al., Latest advances of microbial production of 2, 3-butanediol, Progress in Chemistry, 24, 11, pp. 2268-2276, (2012)

[10]

Wang M., Fu J., Zhang X.Y., Et al., Metabolic engineering of Bacillus subtilis for enhanced production of acetoin, Biotechnology Letters, 34, 10, pp. 1877-1885, (2012)

← 1 2 →