Nondegenerate two-photon absorption properties of a newly synthesized carbazole derivative

被引：0
作者：
Chen S. [1 ,3 ]
Zheng Y.-C. [1 ,4 ]
Zheng M.-L. [1 ]
Dong X.-Z. [1 ]
Jin F. [1 ]
Zhao Z.-S. [1 ]
Duan X.-M. [2 ]
机构：
[1] Laboratory of Organic NanoPhotonics and Laboratory of Bio-Inspired Smart Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29 Zhongguancun East Road, Beijing
[2] Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, No. 266 Fangzheng Ave Shuitu Technology Development Zone, Beibei District, 400714, Chongqing
[3] College of Physics and Engineering, Zhengzhou University, Zhengzhou
[4] Research Institute of Chemical Defense, P.O. BOX 1043, Beijing
来源：
Journal of Materials Chemistry C | 2017年 / 5卷 / 02期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1039/C6TC04676B
中图分类号：
学科分类号：
摘要：
A two-color two-photon laser scanning microscope (2c2pLSM) has been developed for fluorescence imaging in tissues through the nondegenerate two-photon excitation of fluorescent proteins. However, fluorescent probes have not been widely designed and applied to 2c2pLSM. Here, we have synthesized a new carbazole derivative 3,6-bis(pyridin-4-ylethynyl)-9-methyl-carbazole (BEMC), and investigated its nondegenerate two-photon absorption (ND-TPA) properties. Nondegenerate two-photon excited fluorescence (ND-TPEF) and ND-TPA cross-sections excited by a constant pump wavelength of 800 nm and several probe wavelengths from 485 nm to 650 nm were studied based on the femtosecond pump-probe technique. The fluorescence intensity is linearly dependent on each laser intensity of the two beams, and the dependence of ND-TPEF intensity on time delay between the two lasers is in good agreement with the cross-correlation of the pulse widths of the two lasers. Compared with the corresponding degenerate two-photon absorption (D-TPA) cross-sections for transition to the same excited state via the D-TPA pathway, the ND-TPA cross-sections exhibit at least twofold larger values than the D-TPA case, which is attributed to the effect of intermediate state resonance enhancement (ISRE). This study indicates that the newly synthesized carbazole derivative would serve as a promising fluorescent probe in a two-color two-photon laser scanning microscope (2c2pLSM). © The Royal Society of Chemistry.
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页码：470 / 475
页数：5
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