Detection of tobacco virus by siRNA high-throughput sequencing

被引：0

作者：

Wang, Haojun ^{[1
]}

Wang, Fang ^{[2
]}

Yao, Zhongda ^{[1
]}

Guo, Dongfeng ^{[1
]}

Shu, Junsheng ^{[1
]}

机构：

[1] Technology Center, Anhui Cigarette Industrial Co., Ltd., Hefei

[2] Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei

来源：

Acta Tabacaria Sinica | 2015年 / 21卷 / 03期

关键词：

High throughput sequencing; Small interference RNA; Virus detection;

D O I：

10.16472/j.chinatobacco.2014.043

中图分类号：

学科分类号：

摘要：

Quick detection of tobacco virus strains and identification of new virus were achieved through 2nd generation sequencing technique to perform high throughput sequencing of tobacco siRNA and then screening for RNA virus sequences with bioinformatics methods. Results showed that PVY, CMV, TMV, TVBMV and other virus genomic information were detected in southern part of Anhui tobacco growing areas. Three contigs were identified as partly homologous with Potato leafroll virus, which might be a novel tobacco virus. This research established a new strategy to determine naturally occurred RNA viruses and novel virus in tobacco, and could be applied to virus surveillance in tobacco. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

引用

页码：107 / 110

页数：3

共 4 条

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[3] Dong J.H., Yin Y.Y., Xu X.Y., Et al., First report of Tomato spotted wilt virus in tomato and tobacco in China, Journal of Plant Pathology, 92, 4S, pp. S4.107-S4.122, (2010)
[4] Wu Q., Luo Y., Lu R., Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs, Proc Natl Acad Sci USA, 107, 4, pp. 1606-1611, (2010)

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