Response of soil microbial community structure and function to the sewage leakage: A case study of a 25-year-old cesspool

被引：1
作者：
Wei X. [1 ,2 ,3 ]
Liang J. [1 ,2 ,3 ]
Ning T. [1 ,2 ]
Zhang C. [1 ,2 ]
Wang J. [1 ,2 ]
Tan L. [1 ,2 ]
Shen F. [1 ,2 ]
机构：
[1] Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, No, 31 Fukang Road, Nankai District, Tianjin
[2] Key Laboratory of Rural Toilet and Sewage Treatment Technology, Ministry of Agriculture and Rural Affairs, No. 31 Fukang Road, Nankai District, Tianjin
来源：
Chemosphere | 2024年 / 363卷
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Antibiotic resistance genes; Cesspool; Functional genes; Pathogens; Soil microbial community structure;
D O I：
10.1016/j.chemosphere.2024.142753
中图分类号：
学科分类号：
摘要：
Providing many millions of rural households with decentralized sanitation facilities remains challenging. In undeveloped areas, cesspools have still been widely used due to technologically simple and low-cost. However, the influence of cesspools on the surrounding soil remains unclear. In this study, we investigated the influence of a 25-year-old household cesspool on soil physicochemical factors, microbial community composition and function, pathogens and antibiotic resistance genes (ARGs). Soil at the depth around the sewage liquid level (D70) was mostly disturbed where TOC, NO3–N and TP was increased to 16.8 g/kg, 18.2 mg/kg and 1.02 mg/kg respectively. Correspondingly, the element cycling genes of carbon fixation, methanotrophy, nitrogen fixation, ammonia oxidation, and nitrate reduction etc., were increased at D70. Notably, human derived pathogens such as Enterobacter, Salmonella, Pseudomonas aeruginosa, Klebsiella pneumoniae, Prevotella, and Vibrio were highly enriched by 5–10 folders in D70, indicating the potential health risk to human. Mantel tests suggested that EC, TP, pH, NH3–N and particularly NO3–N are important factors that influence the microbial community and element cycling genes in cesspool-affected soil. Overall, this study revealed the impact of household cesspool leakage on the surrounding soil and provided information for the selection and construction of basic sanitation facilities in poor regions. © 2024 Elsevier Ltd
引用
收藏
相关论文
共 70 条
  • [1] Abascal E., Gomez-Coma L., Ortiz I., Ortiz A., Global diagnosis of nitrate pollution in groundwater and review of removal technologies, Sci. Total Environ., 810, (2022)
  • [2] Abaya L.M., Wiegner T.N., Beets J.P., Colbert S.L., Carlson K.A.M., Kramer K.L., Spatial distribution of sewage pollution on a Hawaiian coral reef, Mar. Pollut. Bull., 130, pp. 335-347, (2018)
  • [3] Adegoke A., Stenstrom T., Cesspits and soakpits, Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Water Pathogen Project), (2019)
  • [4] Anantharaman K., Hausmann B., Jungbluth S.P., Kantor R.S., Lavy A., Warren L.A., Rappe M.S., Pester M., Loy A., Thomas B.C., Banfield J.F., Expanded diversity of microbial groups that shape the dissimilatory sulfur cycle, ISME J., 12, pp. 1715-1728, (2018)
  • [5] Anders R., Chrysikopoulos C.V., Transport of viruses through saturated and unsaturated columns packed with sand, Transport Porous Media, 76, pp. 121-138, (2009)
  • [6] Aw T., Rose J., Jimenez-Cisneros B., Environmental Aspects and Features of Critical Pathogen Groups, (2019)
  • [7] Back J.O., Rivett M.O., Hinz L.B., Mackay N., Wanangwa G.J., Phiri O.L., Songola C.E., Thomas M.A.S., Kumwenda S., Nhlema M., Miller A.V.M., Kalin R.M., Risk assessment to groundwater of pit latrine rural sanitation policy in developing country settings, Sci. Total Environ., 613-614, pp. 592-610, (2018)
  • [8] Barrios R.E., Bartelt-Hunt S.L., Li Y., Li X., Modeling the vertical transport of antibiotic resistance genes in agricultural soils following manure application, Environ. Pollut., 285, (2021)
  • [9] Becker M.W., Collins S.A., Metge D.W., Harvey R.W., Shapiro A.M., Effect of cell physicochemical characteristics and motility on bacterial transport in groundwater, J. Contam. Hydrol., 69, pp. 195-213, (2004)
  • [10] Brown J., Cairncross S., Ensink J.H., Water, sanitation, hygiene and enteric infections in children, Arch. Dis. Child., 98, pp. 629-634, (2013)
1234567