The inhibitory effects of γ-tocotrienol on human colon carcinoma HT-29 cells in vitro

被引：0

作者：

Xu, Weili ^{[1
]}

Zhang, Lanwei ^{[1
]}

Yang, Xing ^{[1
]}

Shan, Yujuan ^{[1
]}

Thavarajah, Pushparajah ^{[2
]}

Song, Wei ^{[1
]}

Dai, Shaochun ^{[3
]}

机构：

[1] College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China

[2] School of Food Systems, North Dakota State University, Fargo 58105, United States

[3] The Third Affiliated Hospital, Harbin Medical University, Harbin 150082, China

来源：

Journal of the Chinese Cereals and Oils Association | 2014年 / 29卷 / 01期

关键词：

Food technology;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To investigate the inhibitory effects and induction of apoptosis on human colon carcinoma cells (HT-29) from γ-tocotrienol, the effect of γ-tocotrienol on cell viability has been investigated by 3-(4.5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method, mitotic index, colony forming experiment and morphological observation; apoptosis has been measured by Hoechst33258 fluorescent staining to observe the morphological observation of apoptosis and DNA ladder to detect DNA damage. The results showed that γ-tocotrienol could obviously inhibit the proliferation of HT-29 cells (P<0.05) with dose-dependent manner. The 50% inhibitory concentration was 35.45 μmol /L (48 h). Morphology observation and DNA ladder showed that γ-tocotrienol could induce apoptosis. The results indicated that γ-tocotrienol can inhibit the growth of human colon carcinoma HT-29 cells through apoptosis induction.

引用

页码：47 / 51