Effect of green tea catechins on vascular cell proliferation and reactive oxygen species

被引：0

作者：

Yang H. ^{[1
]}

Liu Y.-Y. ^{[1
]}

机构：

[1] School of Life Science and Technology, University of Electronic Science and Technology of China

来源：

Dianzi Keji Daxue Xuebao/Journal of the University of Electronic Science and Technology of China | 2011年 / 40卷 / 01期

关键词：

Atherosclerosis; Cell proliferation; Green tea catechins; Reactive oxygen species;

D O I：

10.3969/j.issn.1001-0548.2011.01.028

中图分类号：

学科分类号：

摘要：

One of the most important causes of the atherosclerosis is reactive oxygen species (ROS) to induce lipide overoxidation, which leads to endothelial cells dysfunction and vascular smooth muscle cells (VSMCs) abnormal proliferation. Recent investigations demonstrated that polyphenon has prevention effect in the treatment of atherosclerosis or other vascular diseases. In this paper, endothelial cells (ECs), VSMCs and HL-60 cell (as the abnormal cell model) were used to investigate the effects of green tea catechins on the proliferation. Furthermore, the clearance ability of ROS by catechins was detected by hypoxanthine-xanthineoxidase chemiluminescence method. It was found that the green tea catechins could lightly increase ECs proliferation at the concentration of 1 μg/mL, and green tea catechins of 1 and 3 μg/mL have no obvious effect on VSMCs proliferation. However, green tea catechins of 10 μg/mL significantly inhibited VSMCs proliferation. For HL-60 cells, the cell viability was presented in a dose-dependent manner. Similarly, the ROS clearance ability was also related to the dosage of green tea catechins.

引用

页码：147 / 151

页数：4

共 13 条

[1]

Lusis A.J., Atherosclerosis, Nature, 407, pp. 233-241, (2000)

[2]

Falk E., Pathogenesis of atherosclerosis, J Am Coll Cardiol, 47, (2006)

[3]

Jing Y., Piao A.-D., Study of the plant polyphenon structure and their biological activities, Food and Nutrition in China, 9, pp. 27-29, (2005)

[4]

Jochmann N., Baumann G., Stangl V., Green tea and cardiovascular disease: From molecular targets towards human health, Curr Opin Clin Nutr Metab Care, 11, 6, pp. 758-765, (2008)

[5]

Lu Y.-Z., Song Y., Wu G.-H., Study on the antioxidant activities of grape polyphenols, Food Science, 27, 12, pp. 213-216, (2006)

[6]

de Mejia E.G., Ramirez-Mares M.V., Puangpraphant S., Bioactive components of tea: Cancer, inflammation and behavior, Brain Behav Immun, 23, 6, pp. 721-731, (2009)

[7]

Babu P.V., Liu D., Green tea catechins and cardiovascular health: An update, Curr Med Chem, 15, 18, pp. 1840-1850, (2008)

[8]

Finkel T., Holbrook N.J., Oxidants, oxidative stress and the biology of ageing, Nature, 408, 9, pp. 239-247, (2000)

[9]

Lin J.-M., Qu F., Shan X.-Q., The principles and methods of reactive oxygen species determination, Chinese Journal of Analytical Chemistry, 30, 12, pp. 1507-1514, (2002)

[10]

Kato M., Minakami H., Kuroiwa M., Et al., Superoxide radical generation and mn- and Cu-zn superoxide dismutases activities in human leukemic cells, Hematol Oncol, 21, pp. 11-16, (2003)

← 1 2 →