Effects of Milk Fat Globule Membrane Supplementation to Rats During Pregnancy and Lactation on Bone Growth and Bone Health of Offspring

被引：0

作者：

Han, Lihua ^{[1
,2
]}

Mao, Xueying ^{[1
]}

机构：

[1] College of Food Science and Nutritional Engineering, Key Laboratory of Functional Dairy, Ministry of Education, China Agricultural University, Beijing

[2] Culinary Science and Technology Department, Jiangsu College of Tourism, Yangzhou

来源：

Journal of Food Science and Technology (China) | 2024年 / 42卷 / 05期

基金：

中国国家自然科学基金;

关键词：

bone growth; bone health; early life; maternal nutrition; milk fat globule membrane;

D O I：

10.12301/spxb202200936

中图分类号：

学科分类号：

摘要：

The effects of maternal milk fat globule membrane (MFGM) supplementation on skeletal growth and bone health of offspring were investigated. During pregnancy and lactation, SD female rats were orally administrated with MFGM at a dose of 400 mg / kg. Offspring were weaned at postnatal day 21 and subsequently fed a high-fat diet until postnatal week 14 (adulthood). The longitudinal bone growth parameters of offspring were determined at birth (postnatal day 0), postnatal day 10 and 21. Obesity and bone health state of adult offspring rats were determined at postnatal week 14. Compared with the control group, no significant differences in body weight, body length, tail length, femur length and tibia length of offspring before weaning were found in maternal MFGM supplemented group. Similarly, there were no marked changes in the heights of proliferative and hypertrophic chondrocytes in the growth plate, nor in the expression levels of collagen type 2α (COL2α) and collagen type X (COLX), which are specific to proliferative and hypertrophic chondrocytes, respectively. However, maternal MFGM supplementation during pregnancy and lactation significantly reduced the body weight gain and accumulation of white adipose tissue caused by the high-fat diet in adult offspring compared to the control group. The number of bone marrow adipocytes was notably decreased, while the protein expression of Runx2 and OPG was upregulated significantly, and RANKL expression was downregulated significantly in the MFGM group. Although maternal MFGM supplementation had no significant effect on the longitudinal skeletal growth of pre-weaning offspring, it effectively mitigated the high-fat diet-induced accumulation of bone marrow adipocytes and improved bone metabolism in adult offspring. The study suggested that maternal supplementation with MFGM provided long-term protective effects on bone health of adult offspring. © 2024 Beijing Technology and Business University, Department of Science and Technology. All rights reserved.

引用

页码：33 / 40

页数：7

共 20 条

[1]

ZHENG J, FENG Q Y, ZHENG S, Et al., Maternal nutrition and the developmental origins of osteoporosis in offspring: potential mechanisms and clinical implications [ J ], Experimental Biology and Medicine, 243, 10, pp. 836-842, (2018)

[2]

SHI Y, SABEN J L, HE G X, Et al., Diet-induced metabolic dysregulation in female mice causes osteopenia in adult offspring [ J], Journal of the Endocrine Society, 4, 4, (2020)

[3]

BUCKELS E J, BOLAM S M, TAY M L, Et al., The impact of maternal high-fat diet on bone microarchitecture in offspring, Frontiers in Nutrition, 8, (2021)

[4]

LI T G, YUAN Q C, GONG H, Et al., Gut microbiota mediates the alleviative effect of polar lipids-enriched milk fat globule membrane on obesity-induced glucose metabolism disorders in peripheral tissues in rat dams [J], International Journal of Obesity, 46, 4, pp. 793-801, (2022)

[5]

LI T G, GAO J, DU M, Et al., Milk fat globule membrane supplementation modulates the gut microbiota and attenuates metabolic endotoxemia in high-fat diet-fed mice, Journal of Functional Foods, 47, pp. 56-65, (2018)

[6]

LI T G, GAO J, DU M, Et al., Milk fat globule membrane attenuates high-fat diet-induced obesity by inhibiting adipogenesis and increasing uncoupling protein 1 expression in white adipose tissue of mice, Nutrients, 10, 3, (2018)

[7]

HAN L H, DU M, REN F Z, Et al., Milk polar lipids supplementation to obese rats during pregnancy and lactation benefited skeletal outcomes of male offspring [ J], Molecular Nutrition & Food Research, 65, 15, (2021)

[8]

FERGUSON J W, ATIT R P., A tale of two cities: the genetic mechanisms governing calvarial bone development, Genesis, 57, 1, (2019)

[9]

RAMASWAMY G, KIM H, ZHANG D Y, Et al., Gsα controls cortical bone quality by regulating osteoclast differentiation via cAMP/ PKA and β-catenin pathways, Scientific Reports, 7, (2017)

[10]

SCHELLER E L, KHOURY B, MOLLER K L, Et al., Changes in skeletal integrity and marrow adiposity during high-fat diet and after weight loss, Frontiers in Endocrinology, 7, (2016)

← 1 2 →