Determination of harmine and harmaline in Peganum harmala seeds by high-performance liquid chromatography

被引：0

作者：

Monsef-Esfahani, Hamid Reza ^{[1
]}

Faramarzi, Mohammad Ali ^{[2
]}

Mortezaee, Venus ^{[1
]}

Amini, Mohsen ^{[3
]}

Rouini, Mohammad Reza ^{[4
]}

机构：

[1] Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences

[2] Department of Pharmaceutical Biotechnology, Biotechnology Research Center, Tehran University of Medical Sciences

[3] Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences

[4] Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences

来源：

Journal of Applied Sciences | 2008年 / 8卷 / 09期

关键词：

Harmaline; Harmalol; Harmine; Harmol; HPLC; Peganum harmala;

D O I：

10.3923/jas.2008.1761.1765

中图分类号：

学科分类号：

摘要：

A High-Performance Liquid Chromatography (HPLC) method was developed for determination of harmine, harmaline, harmol and harmalol in the extract of Peganum harmala seeds. The sample preparation was performed using liquid-liquid extraction. Chromatographic separation was achieved with a Tracer Excel 120 ODSA (150×4.6 mm) column, using a mixture of potassium phosphate buffer (10 mM, pH 7.0): acetonitrile (100:30; v/v) as mobile phase, in an isocratic mode at 1.5 mL min-1. UV detection (λ = 330 nm) was used. The calibration curves were linear (r2>0.998) in the concentration range of 0.5-20 μg mL-1 for all analytes. The lower limit of quantification for all analytes was 0.5 μg mL-1. The within and between day precisions in the measurement of QC samples at three tested concentrations were in the range of 0.6-10.2% for all analytes. The HPLC method is able to measure the harmala alkaloids in the plant extract. The method has suitable reproducibility, sensitivity and resolution for routine and accurate use with UV detection. © 2008 Asian Network for Scientific Information.

引用

页码：1761 / 1765

页数：4

共 23 条

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