Changes in the expression of three cold-regulated genes in 'Elsanta' and 'Selvik' strawberry (Fragaria × ananassa) plants exposed to freezing

被引：5

作者：

机构：

[1] Research Institute of Horticulture, Konstytucji 3 Maja 1/3, Skierniewice

来源：

Badek, Bogumi&lstrok;a (bogumila.badek@inhort.pl) | 1600年 / Instytut Warzywnictwa卷 / 22期

关键词：

Cold stress; Gene expression; Strawberry;

D O I：

10.2478/johr-2014-0022

中图分类号：

学科分类号：

摘要：

Cold temperatures in midwinter and late-spring frosts cause severe damages to strawberry plants cultivated in temperate climate regions. Despite the seriousness of the problem, the plant mechanism of defense against cold stress has not been fully elucidated yet, especially in its molecular aspect. The presented investigations were conducted on the cold-susceptible cultivar 'Elsanta' and the cold-tolerant cultivar 'Selvik'. Expression profiles of three genes (CBF4, COR47 and F3H) were determined at three time-points: 0, 6 and 12 weeks after sub-zero treatment at-12 °C. The CBF4 gene was very strongly up-regulated in 'Selvik' plants and the highest value of the transcript level was detected just after the treatment (time-point 0). The F3H transcript in the treated 'Selvik' plants reached the level 4 times higher than in control plants in the 12th week after treatment (time-point 3). In 'Elsanta' plants, the CBF4 and COR47 genes were slightly up-regulated (time-point 2), while the F3H gene showed stability of expression. High positive correlation between the transcript level of COR47 and transcript level of CBF4 genes was observed for both cultivars.

引用

页码：53 / 61

页数：8

共 53 条

[31]

Owens C.L., Thomashow M.F., Hancock J.F., Iezzoni A.F., CBF1 orthologs in sour cherry and strawberry and the heterologous expression of CBF1 in strawberry, J. Am. Soc. Hortic. Sci., 127, pp. 489-494, (2002)

[32]

Puhakainen T., Hess M.W., Makela P., Svensson J., Heino P., Palva E.T., Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis, Plant Mol. Biol., 54, pp. 743-753, (2004)

[33]

Rugienius R., Sasnauskas A., Investigation of strawberry cultivars and hybrid clones, Sodininkyste Ir Daržininkyste, 24, pp. 34-41, (2005)

[34]

Rodrigo J., Spring frosts in deciduous fruit trees-Morphological damage and flower hardiness, Sci. Hort., 85, pp. 155-173, (2000)

[35]

Schwab W., Schaart J.G., Rosati C., Functional molecular biology research in Fragaria, Genetics and Genomics of Rosaceae, 6, pp. 457-486, (2009)

[36]

Shen G., Pang Y., Wu W., Deng Z., Zhao L., Cao Y., Sun X., Tang K., Cloning and characterization of a flavanone 3-hydroxylase gene from, Ginkgo Biloba. Biosci Rep., 26, pp. 19-29, (2006)

[37]

Shokaeva D.B., Injures induced in different strawberry genotypes by winter freeze and their effect on subsequent yield, Plant Breeding, 127, pp. 197-202, (2008)

[38]

Steponkus P.L., Webb M.S., Freeze-induced dehydration and membrane destabilization in plants, Water and Life: Comparative Analysis of Water Relationships at the Organismic, Cellular and Molecular Level, pp. 338-362, (1992)

[39]

Stockinger E.J., Gilmour S.J., Thomashow M.F., Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat /DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit, Proc. Natl. Acad. Sci. USA, 94, pp. 1035-1040, (1997)

[40]

Takahashi M., Hikage T., Yamashita T., Saitoh Y., Endou M., Tsutsumi K.-I., Stress-related proteins are specially expressed under non-stress conditions in the overwinter buds of the gentian plant, Gentiana Triflora. Breed Sci., 56, pp. 39-46, (2006)

← 1 2 3 4 5 6 →