High-yield degumming methods for preparing sericin from cocoons of mulberry and non-mulberry silkworms

被引：1

作者：

Karimazawa M. ^{[1
]}

Urita S. ^{[2
]}

Yamashita T. ^{[3
]}

Suzuki K. ^{[1
]}

机构：

[1] Biococoon Laboratories Inc, Research and Development Center, in collaboration with Morioka City, Iwate University, Ueda 4-3-5, Morioka

[2] Negishi 5-28, Date

[3] Department of Applied Biochemistry, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka

来源：

Journal of Insect Biotechnology and Sericology | 2019年 / 88卷 / 01期

基金：

日本学术振兴会;

关键词：

Degumming; High yield; Mulberry silkworm; Non-mulberry silkworm; Sericin;

D O I：

10.11416/jibs.88.1_001

中图分类号：

学科分类号：

摘要：

We developed high-yield degumming methods for preparing sericin from the cocoons of the mulberry silkworm Bombyx mori and the non-mulberry silkworms of Antheraea yamamai, and Cricula trifenestrata. We achieved sericin yields of 54%-55% from B. mori cocoons extracted in 0.24% NaOH, from A. yamamai cocoons extracted in 0.025% NaOH + 0.475% Na2CO3, and from C. trifenestrata cocoons extracted in 0.5% Na2CO3. In all three extracts, analysis of amino acid compositions indicated a high degree of sericin purity and Tricine-SDS-PAGE analysis indicated degradation to heterogeneous polypeptides. Thus, sericin preparations produced from the cocoons of silkworms by high-yield degumming methods offer promise for biomedical and industrial applications. © 2019, Japanese Society of Sericultural Sciences. All rights reserved.

引用

页码：1 / 6

页数：5

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