Synthesis and characterization of a amphiphilic pluronic-poly(D,L-lactide- co-glycolide) copolymer and their nanoparticles as protein delivery systems

被引：0

作者：

Wu, Yan ^{[1
]}

Che, Fu-Bin ^{[2
]}

Chen, Jiang-Han ^{[2
]}

机构：

[1] National Center for Nanoscience and Technology, Department of Nano-medicine, Nano-biotech Laboratory, Beijing 100080, China

[2] Shanghai Changzheng Hospital, Department of Pharmaceutics, Shanghai 200003, China

来源：

Journal of Applied Polymer Science | 2008年 / 110卷 / 02期

关键词：

A new amphiphilic Pluronic (F68)-PLGA copolymer; which can be used to prepare the stealth or long-circulating nanoparticles; was synthesized with Pluronic (F68); Di-Lactide; and glycolide. The structures of Pluronic (F68)-PLGA copolymer as the products were characterized with infrared spectrometry; nuclear magnetic resonance and their molecular weights were determined by gel permeation chromatography. Two methods; double emulsion (DE) and nanoprecipitation (NP); were employed to fabricate the polymeric nanoparticles. Bovine serum albumin (BSA) was loaded into nanoparticles as a model protein. Influence of the preparation conditions on the nanoparticles size; encapsulation efficiency; and release profile in vitro was investigated. They showed the entrapment efficiency of 42.9-63.4% and the average diameter of 119.1-342.8 nm depending on the fabrication technique of nanoparticles and the type of copolymer. The stability maintenance of BSA in the nanoparticle release in vitro was also measured via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); circular dichroism (CD); and fluorescence spectrometry. The results showed that the new copolymer could load BSA effectively and BSA kept stable after it was released from the nanoparticles. © 2008 Wiley Periodicals; Inc;

D O I：

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学科分类号：

摘要：

Journal article (JA)

引用

页码：1118 / 1128

共 3 条

[1] Preparation and characterization of poly(DL-lactide-co-glycolide) nanoparticles for siRNA delivery
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INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2010, 390 (01) : 70 - 75
[2] Synthesis and Characterization of Doxorubicin-Loaded Poly(Lactide-co-glycolide) Nanoparticles as a Sustained-Release Anticancer Drug Delivery System
Amjadi, I.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 168 (06) : 1434 - 1447
[3] Synthesis and Characterization of Doxorubicin-Loaded Poly(Lactide-co-glycolide) Nanoparticles as a Sustained-Release Anticancer Drug Delivery System
I. Amjadi
M. Rabiee
M. S. Hosseini
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Applied Biochemistry and Biotechnology, 2012, 168 : 1434 - 1447

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