Isolation and purification of alkaloid from Holarrhena antidysenterica Wall.ex A.DC

被引：0

作者：

Wang H. ^{[1
]}

Zhou Y. ^{[1
]}

Uchida T. ^{[2
]}

Mari Y.Y. ^{[2
]}

Qian S. ^{[1
]}

机构：

[1] School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, Liaoning

[2] Graduate School of Agricultural Science, Tohoku University, Sendai

来源：

Huagong Xuebao/CIESC Journal | 2016年 / 67卷

关键词：

Conessine; Extraction; Holarrhena antidysenterica Wall.ex A.DC; NMR; Pharmaceuticals; Silica gel columnchromatography;

D O I：

10.11949/j.issn.0438-1157.20161237

中图分类号：

学科分类号：

摘要：

In this paper, one of the agent of alkaloid was extracted from the dry seeds of Holarrhena antidysenterica Wall.ex A.DC by ethanol, purified by silica gel chromatography and recrystallization.The structures of it was established on the basis of NMR techniques and mass spectrometry.The results showed that the optimum conditions of extraction were as follows: the Holarrhena antidysenterica Wall.ex A.DC was extracted under 60℃ for 6 h by 70% ethanol, and the getting rate of total alkaloid reached to 0.244%.By silica gel chromatography and recrystallization, the purity of one sample of compound 2 reached to 94.89%.The compound 2 was obtained as white crystal and its molecular formula C24H40N2was established by mass spectrometry (m/z 356.59 [M+H]+).From the NMR data, the structure of compound 2 was deduced as conessine and its 13C NMR data were consistent with those of literature. © All Right Reserved.

引用

页码：191 / 196

页数：5

共 18 条

[1]

Fotie J., Bohle D.S., Leimanis M.L., Et al., Lupeol long-chain fatty acid esters with antimalarial activity from Holarrhena floribunda, J. Nat. Prod., 69, 1, pp. 62-67, (2006)

[2]

Tuntiwachuttikul P., Pootaengon Y., Phansa P., Et al., Constituents of the leaves of Holarrhena pubescens, Fitoterapia, 78, 3, pp. 271-273, (2007)

[3]

Nwodo N.J., Brun R., Osadebe P.O., In vitro and in vivo evaluation of the antitrypanosomal activity of fractions of Holarrhena africana, J. Ethnopharmacology, 113, 3, pp. 556-559, (2007)

[4]

Kavitha D., Niranjali S., Inhibition of enteropathogenic Escherichia coli adhesion on host epithelial cells by Holarrhena antidysenterica(L.)WALL, Phytother. Res., 23, 9, pp. 1229-1236, (2009)

[5]

Wu X.J., Jin C., Pharmacognostical identification of Mongolian medicine Holarrhena antidysenterica Wall. ex A. DC, Chinese Journal of Ethnomedicine and Ethnopharmacy, 49, 2, pp. 111-112, (2001)

[6]

Guo L., Kang W.Y., Chinese Medicinal Chemistry, (2015)

[7]

Geng J.F., Qian S.R.G.L., Wang H.Y., Et al., Extraction technology and bacteriostasis of active substance from semen Holarrhenae, Food Science and Technology, 32, 7, pp. 145-147, (2007)

[8]

Zhao X.M., Li R.J., Zhou C., Et al., Separation and purification of deoxynivalenol(DON)mycotoxin from wheat culture using a simple two-step silica gel column chromatography, Journal of Integrative Agriculture, 15, 3, pp. 694-701, (2016)

[9]

Hu H.C., Cui Y.D., Synthesis and purification of PCBM, CIESC Journal, 66, pp. 461-465, (2015)

[10]

Gaurav M.D., Hemant D.U., Quantification of quercetin and rutin from Benincasa hispida seeds and Carissa congesta roots by high-performance thin layer chromatography and high-performance liquid chromatography, Pharmacognosy Research, 8, 1, pp. 37-42, (2016)

← 1 2 →