Low-concentration imiquimod treatment promotes enhanced skin barrier functions through epidermal melanization reaction regulation

被引：0

作者：

Lin T.-K. ^{[1
,2
]}

Tsai C.-L. ^{[3
]}

Tsai B.C.-K. ^{[3
]}

Kuo C.-H. ^{[4
,5
,6
]}

Ho T.-J. ^{[7
,8
,9
]}

Hsieh D.J.-Y. ^{[10
,11
]}

Kuo W.-W. ^{[12
,13
,14
]}

Huang C.-Y. ^{[3
,15
,16
,17
,18
]}

机构：

[1] Department of Dermatology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien

[2] School of Medicine, Tzu Chi University, Hualien

[3] Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien

[4] Institute of Sports Sciences, University of Taipei, Taipei

[5] Laboratory of Exercise Biochemistry, Institute of Sports Sciences, University of Taipei, Tianmu Campus, Taipei

[6] Department of Kinesiology and Health Science, College of William and Mary, Williamsburg, VA

[7] Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien

[8] Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien

[9] School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Hualien

[10] School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung

[11] Clinical Laboratory, Chung Shan Medical University Hospital, Taichung

[12] Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung

[13] Ph.D. Program for Biotechnology Industry, China Medical University, Taichung

[14] School of Pharmacy, China Medical University, Taichung

[15] Graduate Institute of Biomedical Sciences, China Medical University, Taichung

[16] Department of Medical Research, China Medical University Hospital, China Medical University, Taichung

[17] Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung

[18] Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien

来源：

Environmental Toxicology | 2024年 / 39卷 / 09期

关键词：

dynamin; 3; imiquimod; melanin synthesis; melanization; protein kinase C eta type; skin barrier;

D O I：

10.1002/tox.24332

中图分类号：

学科分类号：

摘要：

The primary function of the skin is to form a mechanical, permeability, antimicrobial, and ultraviolet radiation barrier, which is essential for maintaining physiological homeostasis. Our previous studies demonstrated that cutaneous pigmentation could promote skin barrier function in addition to providing anti-ultraviolet irradiation defense. The present study aimed to develop a new regimen that enhances skin barrier function by regulating skin pigmentation using low-concentration imiquimod. Results showed that topical application of low-concentration imiquimod effectively induced skin hyperpigmentation in the dorsal skin and external ear of mice without inducing inflammatory cell infiltration. An in vitro study also revealed that low-concentration imiquimod did not induce any cytotoxic effects on melanoma cells but triggered excessive melanin synthesis. In coculture systems, low-concentration imiquimod was noted to increase tyrosinase activity in a broader cellular context, revealing the potential role of neighboring cells in melanin production. The next-generation sequencing result indicated that PKCη and Dnm3 might regulate melanin synthesis and release during imiquimod treatment. Overall, our study presents new insights into the regulation of melanin production by low-concentration imiquimod, both in a mice model and cultured cells. Furthermore, our study highlights the potential benefits of imiquimod in promoting melanin synthesis without causing skin disruptions or inducing inflammation, validating its potential to serve as a method for enhancing skin barrier functions by regulating the epidermal melanization reaction. © 2024 Wiley Periodicals LLC.

引用

页码：4360 / 4371