Screening and identification of lipase-producing marine microorganisms and study of enzymatic properties

被引：0
作者：
Zhou, Zhimin [1 ,2 ]
Lin, Juan [1 ,2 ]
Lin, Xianju [1 ,2 ]
Wang, Qiaohuang [1 ,2 ]
Ye, Xiuyun [1 ,2 ]
机构：
[1] College of Biological Science and Engineering, Fuzhou University, Fuzhou
[2] Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou
来源：
Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 11期
关键词：
Burkholderiacepacia; Characterization; Lipase; Marine microorganisms;
D O I：
10.16429/j.1009-7848.2015.11.010
中图分类号：
学科分类号：
摘要：
Objective: Lipase-producing marine microorganisms were isolated and identified, and the enzymatic properties were studied. Methods: Lipase-producing marine microorganisms were isolated with selective mediacontained olive oil as the only source of carbon. The objective strain was identified according to the sequence analysis of 16S rDNA, and the enzymatic properties were studied. Results: Twenty-twolipase-producing marine microorganisms were isolated from five samples, such as the intestinal contents of eeland soil samples. The lipase produced by strain ZF-16 had the highest activity to catalyze glycerolysis of oil. Strain ZF-16 was identified as Burkholderiacepacia according to its morphology characteristics and the sequence of 16S rDNA. The optimal pH and temperature of the lipase were pH 9.0 and 75℃, respectively. Activity of lipase was relatively stable at 40℃ and pH 5.0-10.0. Besides, the activity of lipase wasactivated by 1 mmol/L Na+ and K+, and strongly inhibited by Zn2+. The lipase could degrade substrates with different chain length from C4 to C18, especially tricaprylin (C8). Conclusion: A lipase-producing marine microorganism was successfully isolated and identified as Burkholderiacepacia. The results showed that the lipase was a kind of alkaline lipases resisting to high temperature, and had degradation effects on substrates with different chain length from C4 to C18. Therefore, this lipase could be used in food baking, pulp and paper industryand waste-paper deinking. © 2015, Chinese Institute of Food Science and Technology. All right reserved.
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页码：62 / 70
页数：8
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