RANTES Produced by Actiated Lymphocytes Costimulated by Anti-CD3 mAb and Anti-CD28 mAb

Purpose: To investigate the changes of regulated upon activation, normal T cell expressed and secreted (RANTES) level produced by activated lymphocytes costimulated by CD28 pathway and the effects of Cyclosporin A(CsA). Methods: Human peripheral lymphocytes separated by Ficoll were divided into four groups: anti-CD3mAb group, anti-CD28mAb group, CD28 costimulation group and control group. CD28 costimulation group was treated by different concentrations of PDTC or CsA. RANTES levels were detected by ELISA and CD25 and CCR5 expression of lymphocytes were detected by FCM. Results: RANTES concentration was significantly higher in CD28 costimulation group than other group (P < 0.05). RANTES secreted by lymphocytes could be inhibited by PDTC or CsA. CD25 expression was upregulated after lymphocytes costimulated by CD28 pathway, on the contrary, CCR5 was significantly downregulated (P < 0.05). Conclusions: CD28 costimulating lymphocytes play a important role on RANTES generation which regulated by NF-κB pathway. Lymphocytes producing RANTES and their reaction to RANTES were both related to CD28 pathway. Inhibiting the secretion of RANTES may be one of the important immunosuppressive mechanisms of CsA.