To assess the ultimate bearing capacity of the steel-concrete composite deck slab subject to highway load, ultimate bearing capacity tests were carried out. According to Saint Venant Principle, by taking the part steel-concrete deck slab in column area with positive and negative bending moments as finite element models of the actual bridge, the study focused on the ultimate bearing capacity of the steel-concrete deck slab in column area with positive and negative bending moments for 2 composite deck slab specimens (model A and model B). The results show that tensile failure has occurred in the concrete slab of model A. The upper and lower flange plates of I beam, web plate and longitudinal reinforcement have not yielded under ultimate load, but steel bottom plate, PBL and lateral reinforcement have yielded before failure. The failure load of model A is 1750 kN. As for model B, concrete slab has compression failure, PBL has yielded before failure, and the failure load of model B is 2200 kN. Research results provide reference for design calculation of the ultimate bearing capacity of steel-concrete composite deck slab.