Quantification of DNA in simple eukaryotic cells using Fourier transform infrared spectroscopy

被引：31
作者：
机构：
[1] Centre for Biospectroscopy, School of Chemistry, Monash University, 3800, VIC
[2] Australian Synchrotron, Clayton, VIC 3168
来源：
Wood, B.R. (bayden.wood@monash.edu) | 1600年 / Wiley-VCH Verlag卷 / 06期
关键词：
Cell; DNA; Erythrocyte; FTIR; Hydration;
D O I：
10.1002/jbio.201200112
中图分类号：
学科分类号：
摘要：
A technique capable of detecting and monitoring nucleic acid concentration offers potential in diagnosing cancer and further developing an understanding of the biochemistry of disease. The application of Fourier transform infrared (FTIR) spectroscopy has previously been hindered by the supposed non-Beer-Lambert absorption behavior of DNA in intact cells making elucidation of the DNA bands difficult. We use known composition DNA/hemoglobin standards to successfully estimate the DNA content in avian erythrocyte nuclei (44.2%) and intact erythrocytes (12.8%). Furthermore we demonstrate that the absorption of cellular DNA does follow the Beer-Lambert Law and highlights the role of conformation and hydration in FTIR spectroscopy of biological samples. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：775 / 784
页数：9
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